The CRISPR-Cas9 genome-editing system is a part of the adaptive immune system in
archaea and bacteria to defend against invasive nucleic acids from phages and plasmids …

CRISPR/Cas technology has revolutionized the fields of the genome-and epigenome-
editing by supplying unparalleled control over genomic sequences and expression …

Stem cells represent natural units of embryonic development and tissue regeneration.
Embryonic stem (ES) cells, in particular, possess a nearly unlimited self-renewal capacity …

The host range of retroviral vectors including lentiviral vectors can be expanded or altered
by a process known as pseudotyping. Pseudotyped lentiviral vectors consist of vector …

The art and science of gene therapy has received much attention of late. The tragic death of
18-year-old Jesse Gelsinger, a volunteer in a Phase I clinical trial, has overshadowed the …

The capacity to efficiently transduce nondividing cells, shuttle large genetic payloads, and
maintain stable long-term transgene expression are attributes that have brought lentiviral …

A key factor in the success of gene therapy is the development of gene delivery systems that
are capable of efficient gene transfer in a broad variety of tissues, without causing any …

We describe the generation and the characterization of new lentiviral vectors derived from
SIVmac251, a simian immunodeficiency virus (SIV). A methodical approach was used to …

Infection with the SARS-associated coronavirus (SARS-CoV) induces an atypical pulmonary
disease with a high lethality rate. Although the initial SARS epidemic was contained …

Lentiviruses induce a wide variety of pathologies in different animal species. A common
feature of the replicative cycle of these viruses is their ability to target non-dividing cells, a …