Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
[HTML][HTML] Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …
have been combined only recently to facilitate novel experiments and measurements in …
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
WWY Yim, H Yamamoto, N Mizushima - Elife, 2022 - elifesciences.org
Monitoring autophagic flux is necessary for most autophagy studies. The autophagic flux
assays currently available for mammalian cells are generally complicated and do not yield …
assays currently available for mammalian cells are generally complicated and do not yield …
Zebrahub–Multimodal zebrafish developmental atlas reveals the State-transition dynamics of late-vertebrate pluripotent axial progenitors
Elucidating the developmental processes of organisms requires a comprehensive
understanding of cellular lineages in the spatial, temporal, and molecular domains. In this …
understanding of cellular lineages in the spatial, temporal, and molecular domains. In this …
[HTML][HTML] Fast, efficient, and accurate neuro-imaging denoising via supervised deep learning
Volumetric functional imaging is widely used for recording neuron activities in vivo, but there
exist tradeoffs between the quality of the extracted calcium traces, imaging speed, and laser …
exist tradeoffs between the quality of the extracted calcium traces, imaging speed, and laser …
[HTML][HTML] Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
S Takanezawa, T Saitou, T Imamura - Nature Communications, 2021 - nature.com
Two-photon excitation can lower phototoxicity and improve penetration depth, but its narrow
excitation range restricts its applications in light-sheet microscopy. Here, we propose simple …
excitation range restricts its applications in light-sheet microscopy. Here, we propose simple …
Single‐cell analysis reveals dynamic changes of neural cells in developing human spinal cord
Q Zhang, X Wu, Y Fan, P Jiang, Y Zhao, Y Yang… - EMBO …, 2021 - embopress.org
During central nervous system development, neurogenesis and gliogenesis occur in an
orderly manner to create precise neural circuitry. However, no systematic dataset of neural …
orderly manner to create precise neural circuitry. However, no systematic dataset of neural …
Environmentally relevant concentrations of boscalid exposure affects the neurobehavioral response of zebrafish by disrupting visual and nervous systems
L Qian, S Qi, Z Wang, JT Magnuson, DC Volz… - Journal of Hazardous …, 2021 - Elsevier
Boscalid is a persistent fungicide that is frequently detected in surface waters and may be
neurotoxic to aquatic organisms. Herein, we evaluated the effects of environmentally …
neurotoxic to aquatic organisms. Herein, we evaluated the effects of environmentally …
[HTML][HTML] Cerebellar neurodynamics predict decision timing and outcome on the single-trial level
Goal-directed behavior requires the interaction of multiple brain regions. How these regions
and their interactions with brain-wide activity drive action selection is less understood. We …
and their interactions with brain-wide activity drive action selection is less understood. We …
Single-cell biology: beyond the sum of its parts
AF Schier - Nature Methods, 2020 - nature.com
The field of single-cell RNA sequencing (scRNA-seq) has been paired with genomics,
epigenomics, spatial omics, proteomics and imaging to achieve multimodal measurements …
epigenomics, spatial omics, proteomics and imaging to achieve multimodal measurements …