Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

A fundamental strategy of eukaryotic antiviral immunity involves the cGAS enzyme, which
synthesizes 2′, 3′-cGAMP and activates the effector STING. Diverse bacteria contain …

Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Leading CRISPR-Cas technologies employ Cas9 and Cas12 enzymes that generate RNA-
guided dsDNA breaks. Yet, the most abundant microbial adaptive immune systems, Type I …

Mechanistic insights into the role of the human microbiome in the predisposition to and
treatment of disease are limited by the lack of methods to precisely add or remove microbial …

Summary Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA.
First, an RNA-guided complex named Cascade identifies the complementary DNA target …

The generation of genetic diversity via mutagenesis is routinely used for protein engineering
and pathway optimization. Current technologies for random mutagenesis often target either …

Soybean is considered one of the important crops among legumes. Due to high nutritional
contents in seed (proteins, sugars, oil, fatty acids, and amino acids), soybean is used …

Bacteria deploy multiple defenses to prevent mobile genetic element (MGEs) invasion.
CRISPR–Cas immune systems use RNA-guided nucleases to target MGEs, which counter …