Saccharomyces cerevisiae is a widely used microorganism and a greatly popular cell factory
for the production of various chemicals. In order to improve the yield of target chemicals, it is …

Type I CRISPR systems are widespread in bacteria and archaea. The main differences
compared to more widely applied type II systems are multi-effector CASCADE needed for …

The rapid spread of multidrug resistance (MDR), due to abusive use of antibiotics has led to
global health emergency, causing substantial morbidity and mortality. Bacteria attain MDR …

Over the last years, the discovery of various natural and the development of a row of
engineered CRISPR/Cas nucleases have made almost every site of plant genomes …

Efficient metabolic engineering and the development of mitochondrial therapeutics often rely
upon the specific and strong import of foreign proteins into mitochondria. Fusing a protein to …

Prokaryotes have adaptive defence mechanisms that protect them from mobile genetic
elements and viral infection. One defence mechanism is called CRISPR–Cas (clustered …

Cyanobacteria are photosynthetic organisms that have garnered significant recognition as
potential hosts for sustainable bioproduction. However, their complex regulatory networks …

For scientists, engineers, teachers, entre-preneurs, DIY'ers, and other specialists looking to
uncover and unlock the secrets of the bacterial world, CRISPR-based tools may jump out as …

The clustered regularly interspaced short palindromic repeats and their associated proteins
(CRISPR-Cas) system widely occurs in prokaryotic organisms to recognize and destruct …

Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome
editing. We previously developed and utilized a Pseudomonas-specific PEHR system …