The functional properties of proteins in the crystalline state have been investigated over the
past 30 years by a variety of methods, including single crystal polarized absorption …

Publisher Summary This chapter describes oxygen binding to sickle-cell hemoglobin. Sickle-
cell anemia was first recognized as a distinct disease in 1910, but only during the past three …

All of the NMR methods described are sensitive to intracellular polymerization. Currently
none of them are capable of yielding a completely independent measurement of the …

We have used polarized absorbance to observe the process of monomer accretion and
polymer alignment which occurs in domains of sickle hemoglobin that are formed and …

The re-equilibration rate of carbon monoxide binding to hemoglobin S polymers is
determined by time-resolved measurements of linear dichroism spectra. Linear dichroism is …

Huey-Sheng Shieh, 1 Sandro Ghisla, 5 Louise Karle Hanson,-1 Martha L. Ludwig,* and
Christer E. Nordman abstract: The molecular complex lumiflavin-2-aminobenzoic acid …

Sickle cell hemoglobin (HbS) prepared in argon-saturated 1.8 M phosphate buffer was
rapidly mixed with carbon monoxide (CO)-saturated buffer. The binding of CO to the sickle …

We have generalized the double nucleation mechanism of Ferrone et al.(Ferrone, FA, J.
Hofrichter, H. Sunshine, and WA Eaton. 1980. Biophys. J. 32: 361–377; Ferrone, FA, J …

Time-resolved linear dichroism (TRLD) measurements are conducted on gels of sickle cell
hemoglobin following laser photolysis of the carbonyl adduct to monitor religation kinetics to …

The effect of the carbonmonoxy forms of Hb S and Hb A on the solubility and kinetics of the
aggregation of deoxy-Hb S in concentrated phosphate buffer was studied. The delay time …