Since their discovery many decades ago, Pseudomonas putida and related subspecies
have been intensively studied with regard to their potential application in industrial …

In this review, we examine numerous applications of the green fluorescent protein (GFP)
marker gene in environmental microbiology research. The GFP and its variants are reviewed …

An improved method for gene replacement in Pseudomonas aeruginosa was developed.
The method employs several new gene replacement vectors that incorporate (1) the …

An improved method for integration of exogenous DNA fragments at a defined site within the
genome of Pseudomonas aeruginosa was developed. The method relies on two integration …

Escherichia coli (E. coli) has played a pivotal role in the development of genetics and
molecular biology as scientific fields. It is therefore not surprising that synthetic biology (SB) …

While the possible advantages of bioremediation and phytoremediation, by both
recombinant microbes and plants, have been extensively reviewed, the biosafety concerns …

Nitrogen is the most limiting nutritional factor for the growth of plants. Since plants cannot
reduce atmospheric N 2, they require exogenously fixed nitrogen for growth and …

The Flp-FRT site-specific recombination system from Saccharomyces cerevisiae is a
powerful and efficient tool for high-throughput genetic analysis of bacteria in the …

Throughout the 3000 million years that bacteria have been on planet Earth, they have
evolved amazing mechanisms for rapid adaptation to every imaginable type of …

Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes
with xylene oxygenase-and styrene monooxygenase-encoding genes on their …