Neurochemical organization of the Drosophila brain visualized by endogenously tagged neurotransmitter receptors

S Kondo, T Takahashi, N Yamagata, Y Imanishi… - Cell Reports, 2020 - cell.com
Neurotransmitters often have multiple receptors that induce distinct responses in receiving
cells. Expression and localization of neurotransmitter receptors in individual neurons are …

An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination

O Kanca, J Zirin, Y Hu, B Tepe, D Dutta, WW Lin, L Ma… - elife, 2022 - elifesciences.org
Previously, we described a large collection of Drosophila strains that each carry an artificial
exon containing a T2AGAL4 cassette inserted in an intron of a target gene based on …

A single-cell atlas of adult Drosophila ovary identifies transcriptional programs and somatic cell lineage regulating oogenesis

A Jevitt, D Chatterjee, G Xie, XF Wang, T Otwell… - PLOS …, 2020 - journals.plos.org
Oogenesis is a complex developmental process that involves spatiotemporally regulated
coordination between the germline and supporting, somatic cell populations. This process …

Loss-or gain-of-function mutations in ACOX1 cause axonal loss via different mechanisms

H Chung, MF Wangler, PC Marcogliese, J Jo… - Neuron, 2020 - cell.com
Summary ACOX1 (acyl-CoA oxidase 1) encodes the first and rate-limiting enzyme of the
very-long-chain fatty acid (VLCFA) β-oxidation pathway in peroxisomes and leads to H 2 O 2 …

Drosophila melanogaster: a simple genetic model of kidney structure, function and disease

JAT Dow, M Simons, MF Romero - Nature Reviews Nephrology, 2022 - nature.com
Although the genetic basis of many kidney diseases is being rapidly elucidated, their
experimental study remains problematic owing to the lack of suitable models. The fruitfly …

A single cell genomics atlas of the Drosophila larval eye reveals distinct photoreceptor developmental timelines

KK Bollepogu Raja, K Yeung, YK Shim, Y Li… - Nature …, 2023 - nature.com
The Drosophila eye is a powerful model system to study the dynamics of cell differentiation,
cell state transitions, cell maturation, and pattern formation. However, a high-resolution …

De novo variants in FRMD5 are associated with developmental delay, intellectual disability, ataxia, and abnormalities of eye movement

S Lu, M Ma, X Mao, CA Bacino, J Jankovic… - The American Journal of …, 2022 - cell.com
Proteins containing the FERM (four-point-one, ezrin, radixin, and moesin) domain link the
plasma membrane with cytoskeletal structures at specific cellular locations and have been …

State-of-the-art CRISPR for in vivo and cell-based studies in Drosophila

J Zirin, J Bosch, R Viswanatha, SE Mohr, N Perrimon - Trends in Genetics, 2022 - cell.com
For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful
model organism for biological and biomedical research due to its many genetic and …

Bi-allelic variants in INTS11 are associated with a complex neurological disorder

B Tepe, EL Macke, M Niceta, MW Hubshman… - The American Journal of …, 2023 - cell.com
The Integrator complex is a multi-subunit protein complex that regulates the processing of
nascent RNAs transcribed by RNA polymerase II (RNAPII), including small nuclear RNAs …

Single‐cell RNA sequencing in Drosophila: Technologies and applications

H Li - Wiley Interdisciplinary Reviews: Developmental …, 2021 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) has emerged as a powerful tool for investigating
cell states and functions at the single‐cell level. It has greatly revolutionized transcriptomic …