2. INTRODUCTION The investigation of many fundamental processes in life sciences
requires straightforward tools for fast, sensitive, reliable, and reproducible detection of …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic
measurements of signaling events inside living cells, including detection of protein-protein …

Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

Quantum dots are semiconductor nanocrystals that have broad excitation spectra, narrow
emission spectra, tunable emission peaks, long fluorescence lifetimes, negligible …

Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

Prostate cancer (PCa) is one of the leading cancers in men in the USA. Lack of experimental
tools that predict therapy response is one of the limitations of current therapeutic regimens …

The impact of great scientific insights can very often only be measured decades later.
Theodor Fçrster's theory of Fçrster (or fluorescence) resonance energy transfer (FRET) is …

The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is
yielding fluorophores with exceptional characteristics for live‐cell imaging. In particular, the …