Förster Resonance Energy Transfer (FRET) is a great tool for cell biologists to investigate
molecular interactions in live specimens. FRET is a distance‐dependent phenomenon …

We utilize a cost-effective frequency-domain fluorescence lifetime imaging microscope to
measure the phase lifetime of mTFP1 in mTFP1-mVenus fluorescence resonance energy …

Lipid domains are part of the current description of cell membranes and their involvement in
many fundamental cellular processes is currently acknowledged. However, their study in …

Multiphoton fluorescence lifetime imaging provides an excellent tool for imaging deep within
plant tissues while providing a means to distinguish between fluorophores with high spatial …

In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell
imaging will be introduced, along with methods to record and analyze FLIM data. Relevant …

This paper proposes a new blind end-member and abundance extraction (BEAE) method for
multispectral fluorescence lifetime imaging microscopy (m-FLIM) data. The chemometrical …

We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging
microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously …

Human CO 2 respiration requires rapid conversion between CO 2 and HCO 3−. Carbonic
anhydrase II facilitates this reversible reaction inside red blood cells, and band 3 [anion …

Signal transduction processes are a necessary component of multicellular life, and their
dysregulation is the basis for a host of syndromes and diseases. Thus, it is imperative that …

Live-cell microscopy is now routinely used to monitor the activities of the genetically
encoded biosensor proteins that are designed to directly measure specific cell signaling …