Detecting RNA–protein interactions with EGFP‐cy3 FRET by acceptor photobleaching
SR Alam, MS Mahadevan, A Periasamy - Current Protocols, 2023 - Wiley Online Library
Förster Resonance Energy Transfer (FRET) is a great tool for cell biologists to investigate
molecular interactions in live specimens. FRET is a distance‐dependent phenomenon …
molecular interactions in live specimens. FRET is a distance‐dependent phenomenon …
Linear behavior of the phase lifetime in frequency-domain fluorescence lifetime imaging of FRET constructs
We utilize a cost-effective frequency-domain fluorescence lifetime imaging microscope to
measure the phase lifetime of mTFP1 in mTFP1-mVenus fluorescence resonance energy …
measure the phase lifetime of mTFP1 in mTFP1-mVenus fluorescence resonance energy …
Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
AEP Bastos, S Scolari, M Stöckl, RFM de Almeida - Methods in enzymology, 2012 - Elsevier
Lipid domains are part of the current description of cell membranes and their involvement in
many fundamental cellular processes is currently acknowledged. However, their study in …
many fundamental cellular processes is currently acknowledged. However, their study in …
Multiphoton fluorescence lifetime imaging shows spatial segregation of secondary metabolites in Eucalyptus secretory cavities
AM Heskes, CN Lincoln, JQD Goodger… - Journal of …, 2012 - Wiley Online Library
Multiphoton fluorescence lifetime imaging provides an excellent tool for imaging deep within
plant tissues while providing a means to distinguish between fluorophores with high spatial …
plant tissues while providing a means to distinguish between fluorophores with high spatial …
Quantitative live cell FLIM imaging in three dimensions
A Le Marois, K Suhling - Multi-Parametric Live Cell Microscopy of 3D …, 2017 - Springer
In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell
imaging will be introduced, along with methods to record and analyze FLIM data. Relevant …
imaging will be introduced, along with methods to record and analyze FLIM data. Relevant …
Blind end-member and abundance extraction for multispectral fluorescence lifetime imaging microscopy data
O Gutierrez-Navarro… - IEEE journal of …, 2013 - ieeexplore.ieee.org
This paper proposes a new blind end-member and abundance extraction (BEAE) method for
multispectral fluorescence lifetime imaging microscopy (m-FLIM) data. The chemometrical …
multispectral fluorescence lifetime imaging microscopy (m-FLIM) data. The chemometrical …
PIE-FLIM measurements of two different FRET-based biosensor activities in the same living cells
CA Reissaus, KH Day, RG Mirmira, KW Dunn… - Biophysical …, 2020 - cell.com
We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging
microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously …
microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously …
[HTML][HTML] Adaptable interaction between aquaporin-1 and band 3 reveals a potential role of water channel in blood CO2 transport
Human CO 2 respiration requires rapid conversion between CO 2 and HCO 3−. Carbonic
anhydrase II facilitates this reversible reaction inside red blood cells, and band 3 [anion …
anhydrase II facilitates this reversible reaction inside red blood cells, and band 3 [anion …
[图书][B] Strategies for multiplexed biosensor imaging to study intracellular signaling networks
Signal transduction processes are a necessary component of multicellular life, and their
dysregulation is the basis for a host of syndromes and diseases. Thus, it is imperative that …
dysregulation is the basis for a host of syndromes and diseases. Thus, it is imperative that …
Monitoring biosensor activity in living cells with fluorescence lifetime imaging microscopy
Live-cell microscopy is now routinely used to monitor the activities of the genetically
encoded biosensor proteins that are designed to directly measure specific cell signaling …
encoded biosensor proteins that are designed to directly measure specific cell signaling …