Cryoprotectant toxicity: facts, issues, and questions

BP Best - Rejuvenation research, 2015 - liebertpub.com
High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during
cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become …

Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity?

M Awan, I Buriak, R Fleck, B Fuller, A Goltsev… - Regenerative …, 2020 - Taylor & Francis
Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since
the early history of cryopreservation. It has been used for decades in many thousands of cell …

Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes

S Tunçer, R Gurbanov, I Sheraj, E Solel, O Esenturk… - Scientific reports, 2018 - nature.com
Dimethyl sulfoxide (DMSO) is a small molecule with polar, aprotic and amphiphilic
properties. It serves as a solvent for many polar and nonpolar molecules and continues to be …

Anthelmintic Potential of Thymoquinone and Curcumin on Fasciola gigantica

R Ullah, A Rehman, MF Zafeer, L Rehman, YA Khan… - PloS one, 2017 - journals.plos.org
Fasciolosis an economically important global disease of ruminants in the temperate and
tropical regions, caused by Fasciola hepatica and F. gigantica, respectively, also poses a …

Use of cryoprotectors and antioxidants in sturgeon semen cryopreservation

MN Kolyada, VP Osipova, NT Berberova - Cryobiology, 2023 - Elsevier
In recent years, the possibility of increasing the low cryoresistance of sturgeon sperm by
using antioxidants in basic cryoprotective media has been studied. The goal of this work was …

An overview of NRF2-activating compounds bearing α, β-unsaturated moiety and their antioxidant effects

MC Egbujor, B Buttari, E Profumo… - International Journal of …, 2022 - mdpi.com
The surge of scientific interest in the discovery of Nuclear Factor Erythroid 2 (NFE2)-Related
Factor 2 (NRF2)-activating molecules underscores the importance of NRF2 as a therapeutic …

[HTML][HTML] A dose-dependent effect of dimethyl sulfoxide on lipid content, cell viability and oxidative stress in 3T3-L1 adipocytes

PV Dludla, B Jack, A Viraragavan, C Pheiffer… - Toxicology Reports, 2018 - Elsevier
Dimethyl sulfoxide (DMSO) is an effective solvent and cytoprotectant agent that can induce
diverse actions in experimental settings, ranging from metabolic stress to cytotoxic effects …

Measuring and suppressing the oxidative damage to DNA during Cu (I)-catalyzed azide–alkyne cycloaddition

GR Abel Jr, ZA Calabrese, J Ayco, JE Hein… - Bioconjugate …, 2016 - ACS Publications
We have used the quantitative polymerase chain reaction (qPCR) to measure the extent of
oxidative DNA damage under varying reaction conditions used for copper (I)-catalyzed click …

Phenotypic screening with oleaginous microalgae reveals modulators of lipid productivity

AK Franz, MA Danielewicz, DM Wong… - ACS chemical …, 2013 - ACS Publications
Here we describe the first phenotypic screening with microalgae to study lipid metabolism
and to discover organic small molecules as chemical triggers that increase growth and lipid …

[HTML][HTML] Evaluation of the use of imaging parameters for the detection of compound-induced hepatotoxicity in 384-well cultures of HepG2 cells and cryopreserved …

H Garside, KF Marcoe, J Chesnut-Speelman… - Toxicology in Vitro, 2014 - Elsevier
Drug-induced liver injury (DILI) is a major cause of failed drug development, withdrawal and
restricted usage. Therefore screening assays which aid selection of candidate drugs with …