Recent advances in crop transformation technologies

Z Chen, JM Debernardi, J Dubcovsky, A Gallavotti - Nature plants, 2022 - nature.com
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

The emerging role of nanotechnology in plant genetic engineering

HJ Squire, S Tomatz, E Voke… - Nature Reviews …, 2023 - nature.com
Genetic engineering to improve the capabilities of plants is essential given climate change
and population growth pressures. Current manipulation methods are laborious and species …

[HTML][HTML] Plant grafting: Molecular mechanisms and applications

M Feng, F Augstein, A Kareem, CW Melnyk - Molecular Plant, 2024 - cell.com
People have grafted plants since antiquity for propagation, to increase yields, and to
improve stress tolerance. This cutting and joining of tissues activates an incredible …

DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration

S Najafi, E Bertini, E D'Incà, M Fasoli… - Horticulture …, 2023 - academic.oup.com
CRISPR/Cas9 genome editing technology can overcome many limitations of traditional
breeding, offering enormous potential for crop improvement and food production. Although …

Biotechnological advances to improve abiotic stress tolerance in crops

MA Villalobos-López, A Arroyo-Becerra… - International Journal of …, 2022 - mdpi.com
The major challenges that agriculture is facing in the twenty-first century are increasing
droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate …

Plant protoplasts in the age of synthetic biology

I Reyna-Llorens, M Ferro-Costa… - Journal of experimental …, 2023 - academic.oup.com
Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …

Regeneration of non-chimeric plants from DNA-free edited grapevine protoplasts

S Scintilla, U Salvagnin, L Giacomelli… - Frontiers in Plant …, 2022 - frontiersin.org
The application of New Breeding Techniques (NBTs) in Vitis vinifera is highly desirable to
introduce valuable traits while preserving the genotype of the elite cultivars. However, a …

Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2

S Biswas, NJ Wahl, MJ Thomson, JM Cason… - International Journal of …, 2022 - mdpi.com
The cultivated peanut (Arachis hypogaea L.) is a legume consumed worldwide in the form of
oil, nuts, peanut butter, and candy. Improving peanut production and nutrition will require …

Applications of in vitro tissue culture technologies in breeding and genetic improvement of wheat

A Wijerathna-Yapa, V Ramtekey, B Ranawaka… - Plants, 2022 - mdpi.com
Sources of new genetic variability have been limited to existing germplasm in the past.
Wheat has been studied extensively for various agronomic traits located throughout the …