Multidrug resistant bacteria are a global threat for human and animal health. However, they
are only part of the problem of antibiotic failure. Another bacterial strategy that contributes to …

In contrast to lesions produced within the DNA helix by chemical damage, mismatched base
pairs arise during the course of normal DNA metabolism. Enzyme systems that recognize …

Knowledge of the rate and nature of spontaneous mutation is fundamental to understanding
evolutionary and molecular processes. In this report, we analyze spontaneous mutations …

Biofilm has garnered a lot of interest due to concerns in various sectors such as public
health, medicine, and the pharmaceutical industry. Biofilm-producing bacteria show a …

We describe the construction of six strains of Escherichia coli with different mutations at the
same coding position in the lacZ gene, which specifies the active site glutamic acid residue …

Mutations in the rpoB gene of Escherichia coli result in resistance to the antibiotic rifampicin
(Rifr) by altering the β subunit of RNA polymerase. Previous studies have identified 39 …

We have determined the DNA sequence changes in 487 spontaneous mutations in the N-
terminal part of the lacI gene in mutH, mutL, and mutS strains of Escherichia coli. These …

Mutators are cells that have a higher mutation rate than the wild type. Such mutators have
been extensively studied in bacteria, and this has led to the elucidation of a number of …

We have used a strain with an altered lacZ gene, which reverts to wild type via only certain
transversions, to detect transversion-specific mutators in Escherichia coli. Detection relied …

To evaluate the substrate specificity of methyl-directed mismatch repair in Escherichia coli
extracts, we have constructed a set of DNA heteroduplexes, each of which contains one of …