[HTML][HTML] 3D cell culture systems: tumor application, advantages, and disadvantages
O Habanjar, M Diab-Assaf, F Caldefie-Chezet… - International journal of …, 2021 - mdpi.com
The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long
been used in cancer research. However, this system cannot be fully translated into clinical …
been used in cancer research. However, this system cannot be fully translated into clinical …
[HTML][HTML] Challenges of applying multicellular tumor spheroids in preclinical phase
SJ Han, S Kwon, KS Kim - Cancer cell international, 2021 - Springer
Abstract The three-dimensional (3D) multicellular tumor spheroids (MCTs) model is
becoming an essential tool in cancer research as it expresses an intermediate complexity …
becoming an essential tool in cancer research as it expresses an intermediate complexity …
Biofabrication strategies for 3D in vitro models and regenerative medicine
Organs are complex systems composed of different cells, proteins and signalling molecules
that are arranged in a highly ordered structure to orchestrate a myriad of functions in our …
that are arranged in a highly ordered structure to orchestrate a myriad of functions in our …
[HTML][HTML] Modeling neoplastic disease with spheroids and organoids
Cancer is a complex disease in which both genetic defects and microenvironmental
components contribute to the development, progression, and metastasization of disease …
components contribute to the development, progression, and metastasization of disease …
Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy
Multicellular spheroids are three dimensional in vitro microscale tissue analogs. The current
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …
Matrigel: from discovery and ECM mimicry to assays and models for cancer research
G Benton, I Arnaoutova, J George, HK Kleinman… - Advanced drug delivery …, 2014 - Elsevier
The basement membrane is an important extracellular matrix that is found in all epithelial
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
Three-dimensional printing of Hela cells for cervical tumor model in vitro
Advances in three-dimensional (3D) printing have enabled the direct assembly of cells and
extracellular matrix materials to form in vitro cellular models for 3D biology, the study of …
extracellular matrix materials to form in vitro cellular models for 3D biology, the study of …
[HTML][HTML] Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation
M Vinci, S Gowan, F Boxall, L Patterson… - BMC biology, 2012 - Springer
Background There is overwhelming evidence that in vitro three-dimensional tumor cell
cultures more accurately reflect the complex in vivo microenvironment than simple two …
cultures more accurately reflect the complex in vivo microenvironment than simple two …
Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture
A cell spheroid is a three-dimensional (3D) aggregation of cells. Synthetic, in-vitro spheroids
provide similar metabolism, proliferation, and species concentration gradients to those found …
provide similar metabolism, proliferation, and species concentration gradients to those found …
Multicellular tumor spheroids: an underestimated tool is catching up again
F Hirschhaeuser, H Menne, C Dittfeld, J West… - Journal of …, 2010 - Elsevier
The present article highlights the rationale, potential and flexibility of tumor spheroid mono-
and cocultures for implementation into state of the art anti-cancer therapy test platforms …
and cocultures for implementation into state of the art anti-cancer therapy test platforms …