A microtubule bestiary: structural diversity in tubulin polymers
S Chaaban, GJ Brouhard - Molecular biology of the cell, 2017 - Am Soc Cell Biol
Microtubules are long, slender polymers of αβ-tubulin found in all eukaryotic cells. Tubulins
associate longitudinally to form protofilaments, and adjacent protofilaments associate …
associate longitudinally to form protofilaments, and adjacent protofilaments associate …
Molecular paleontology and complexity in the last eukaryotic common ancestor
VL Koumandou, B Wickstead, ML Ginger… - Critical reviews in …, 2013 - Taylor & Francis
Eukaryogenesis, the origin of the eukaryotic cell, represents one of the fundamental
evolutionary transitions in the history of life on earth. This event, which is estimated to have …
evolutionary transitions in the history of life on earth. This event, which is estimated to have …
Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography
FR Wagner, R Watanabe, R Schampers, D Singh… - Nature protocols, 2020 - nature.com
Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
achieve near-atomic-resolution structures of biochemically isolated macromolecular …
Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography
Cryo-electron tomography (CET) is ideally suited for bridging the resolution gap between
molecular and cellular structural studies. However, CET is limited to a sample thickness …
molecular and cellular structural studies. However, CET is limited to a sample thickness …
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag
MT Swulius, GJ Jensen - Journal of bacteriology, 2012 - Am Soc Microbiol
Based on fluorescence microscopy, the actin homolog MreB has been thought to form
extended helices surrounding the cytoplasm of rod-shaped bacterial cells. The presence of …
extended helices surrounding the cytoplasm of rod-shaped bacterial cells. The presence of …
Prokaryotic cytoskeletons: protein filaments organizing small cells
J Wagstaff, J Löwe - Nature Reviews Microbiology, 2018 - nature.com
Most, if not all, bacterial and archaeal cells contain at least one protein filament system.
Although these filament systems in some cases form structures that are very similar to …
Although these filament systems in some cases form structures that are very similar to …
Cellular electron cryotomography: toward structural biology in situ
CM Oikonomou, GJ Jensen - Annual review of biochemistry, 2017 - annualreviews.org
Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …
Nanoparticle-assisted tubulin assembly is environment dependent
M Unnikrishnan, Y Wang, M Gruebele… - Proceedings of the …, 2024 - pnas.org
Nanomaterials acquire a biomolecular corona upon introduction to biological media, leading
to biological transformations such as changes in protein function, unmasking of epitopes …
to biological transformations such as changes in protein function, unmasking of epitopes …
Six subgroups and extensive recent duplications characterize the evolution of the eukaryotic tubulin protein family
P Findeisen, S Mühlhausen… - Genome biology and …, 2014 - academic.oup.com
Tubulins belong to the most abundant proteins in eukaryotes providing the backbone for
many cellular substructures like the mitotic and meiotic spindles, the intracellular …
many cellular substructures like the mitotic and meiotic spindles, the intracellular …
Challenges and triumphs in cryo-electron tomography
RK Hylton, MT Swulius - Iscience, 2021 - cell.com
Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high.
Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to …
Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to …