Published methods for imaging and quantitatively analyzing morphological changes in
neuronal axons have serious limitations because of their small sample sizes, and their time …

Nanofabrication can help us to emulate natural intelligence. Forward-engineering brain
gained enormous momentum but still falls short in human neurodegenerative disease …

Cells isolated from their native tissues and cultured in vitro face different selection pressures
than those cultured in vivo. These pressures induce a profound transformation that reshapes …

Primary neurons are difficult to cultivate because they are often part of a complex tissue, and
synaptically connected to numerous other cell types. These circumstances often prevent us …

Cues in the micro-environment are key determinants in the emergence of complex cellular
morphologies and functions. Primary among these is the presence of neighboring cells that …

Three-dimensional neuronal culture systems such as spheroids, organoids, and
assembloids constitute a branch of neuronal tissue engineering that has improved our ability …

Background Organotypic brain slices (OTBS) are an excellent experimental compromise
between the facility of working with cell cultures and the biological relevance of using animal …

ABSTRACT A deeper understanding of the brain and its function remains one of the most
significant scientific challenges. It not only is required to find cures for a plethora of brain …

While microfluidic systems model aspects of metastasis, they are limited to artificially created
tissues of limited complexity. We set out to develop an in vitro model of tumor cell migration …

Corticostriatal cocultures are utilized to recapitulate the cortex-striatum connection in vitro as
a convenient model to investigate the development, function, and regulation of synapses …