Enzymatic production of α-d-galactose 1-phosphate by lactose phosphorolysis

MRM De Groeve, V Depreitere, T Desmet… - Biotechnology letters, 2009 - Springer
Abstract α-d-Galactose 1-phosphate (αGal1 P) is an important building block for the
synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have …

Matryoshka enzyme encapsulation: Development of zymoactive hydrogel particles with efficient lactose hydrolysis capability.

MJ Fabra, Z Pérez-Bassart, D Talens-Perales… - Food …, 2019 - Elsevier
This report describes an efficient procedure for enzyme encapsulation and its application for
the hydrolysis of lactose. The enzymatic material that has been developed consists of …

13-Hydroxy-9Z,11E-Octadecadienoic Acid Production by Recombinant Cells Expressing Burkholderia thailandensis 13-Lipoxygenase

DH Sim, KC Shin, DK Oh - Journal of the American Oil Chemists' Society, 2015 - Springer
Linoleate 13-lipoxygenase from Burkholderia thailandensis was expressed in Escherichia
coli for the production of 13-hydroxyoctadecadienoic acid (13-HODE), an antiseptic …

Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized cells of recombinant Escherichia coli expressing the oleate hydratase gene of …

HJ Oh, KC Shin, DK Oh - Biotechnology letters, 2013 - Springer
Recombinant Escherichia coli, expressing the oleate hydratase gene of Stenotrophomonas
maltophilia, was permeabilized by sequential treatments with 0.125 M NaCl and 2 mM …

Synthesis of Cinnamyl Alcohol from Cinnamaldehyde with Bacillus stearothermophilus Alcohol Dehydrogenase as the Isolated Enzyme and in Recombinant E. coli …

A Pennacchio, M Rossi, CA Raia - Applied biochemistry and …, 2013 - Springer
The synthesis of the aroma chemical cinnamyl alcohol (CMO) by means of enzymatic
reduction of cinnamaldehyde (CMA) was investigated using NADH-dependent alcohol …

Tools for characterizing the whole‐cell bio‐oxidation of alkanes at microscale

C Grant, AC da Silva Damas Pinto… - Biotechnology and …, 2012 - Wiley Online Library
This article describes the first reported microwell whole‐cell bioconversion using a water
immiscible substrate that matches the specific activity and yield achieved in a 1.2 L stirred …

L-Carnitine Production Through Biosensor-Guided Construction of the Neurospora crassa Biosynthesis Pathway in Escherichia coli

P Kugler, M Trumm, M Frese… - … in Bioengineering and …, 2021 - frontiersin.org
l-Carnitine is a bioactive compound derived from l-lysine and S-adenosyl-l-methionine,
which is closely associated with the transport of long-chain fatty acids in the intermediary …

Stereoselective synthesis of L-homophenylalanine using the carbamoylase method with in situ racemization via N-acylamino acid racemase

SK Hsu, HH Lo, WD Lin, IC Chen, CH Kao, WH Hsu - Process Biochemistry, 2007 - Elsevier
N-Acylamino acid racemase (NAAAR) gene of Deinococcus radiodurans BCRC12827 was
cloned into expression vector pQE30 to generate pQE-naaar and expressed in recombinant …

Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside

C Yu, H Xu, G Huang, T Chen, G Liu, N Chai… - Applied microbiology …, 2010 - Springer
The main product of the conversion of puerarin by unpermeabilized cells of bacterium
Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241±31.9 µM) …

Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts

BE White, CJ Fenner, MS Smit, STL Harrison - Microbial Cell Factories, 2017 - Springer
Background The regeneration of cofactors and the supply of alkane substrate are key
considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This …