Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes
Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …
[HTML][HTML] Synthetic metabolism for biohalogenation
A Cros, G Alfaro-Espinoza, A De Maria, NT Wirth… - Current opinion in …, 2022 - Elsevier
Highlights•Halogenation is a powerful strategy to tune physicochemical features of organic
molecules.•Synthetic metabolism can be tailored for the incorporation of halogen …
molecules.•Synthetic metabolism can be tailored for the incorporation of halogen …
[HTML][HTML] Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
A nonconventional archaeal fluorinase identified by in silico mining for enhanced fluorine biocatalysis
Fluorinases, the only enzymes known to catalyze the transfer of fluorine to an organic
molecule, are essential catalysts for the biological synthesis of valuable organofluorines …
molecule, are essential catalysts for the biological synthesis of valuable organofluorines …
[HTML][HTML] A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes
Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride
The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
Recursive genome engineering decodes the evolutionary origin of an essential thymidylate kinase activity in Pseudomonas putida KT2440
Thymidylate kinases (TMPKs) play an essential role in DNA biosynthesis across all domains
of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a …
of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a …
Endophytes in lignin valorization: A novel approach
AJ Mattoo, S Nonzom - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Lignin, one of the essential components of lignocellulosic biomass, comprises an abundant
renewable aromatic resource on the planet earth. Although 15%––40% of lignocellulose …
renewable aromatic resource on the planet earth. Although 15%––40% of lignocellulose …
Core and auxiliary functions of one-carbon metabolism in Pseudomonas putida exposed by a systems-level analysis of transcriptional and physiological responses
The soil bacterium Pseudomonas putida is a robust biomanufacturing host that assimilates a
broad range of substrates while efficiently coping with adverse environmental conditions. P …
broad range of substrates while efficiently coping with adverse environmental conditions. P …
From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing
Industrial chemical production largely relies on fossil fuels, resulting in the unavoidable
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …