Long-term stability in biomass and production of terpene indole alkaloids by hairy root culture of Rauvolfia serpentina and cost approximation to endorse commercial …

P Pandey, R Kaur, S Singh, SK Chattopadhyay… - Biotechnology …, 2014 - Springer
The effect of 6 years of cultivation and use of table-sugar (TS) on the biomass/terpene
alkaloid productivities and rol gene expression were studied in a hairy root (HR) clone of …

Hairy roots, callus, and mature plants of Arabidopsis thaliana exhibit distinct glucosinolate and gene expression profiles

A Kastell, I Smetanska, M Schreiner, I Mewis - Plant Cell, Tissue and …, 2013 - Springer
Hairy root cultures transformed with Agrobacterium rhizogenes or undifferentiated callus
cultures are used for production of plant secondary metabolites. Glucosinolates (GS) are a …

蔗糖和光对三裂叶野葛毛状根生长及次生物质产生的影响

何含杰, 梁朋, 施和平 - 生物工程学报, 2005 - cqvip.com
研究了蔗糖浓度和光对固体培养的三裂叶野葛毛状根生长及其总异黄酮和葛根素产生的影响.
结果表明: 在供试的分别添加1%, 3%, 5%, 7% 和9% 蔗糖的MS 固体培养基中, 3 …

Root culture using a mist culture system and estimation of scale‐up feasibility

SH Woo, JM Park, JW Yang - Journal of Chemical Technology …, 1996 - Wiley Online Library
Normal root of Hyoscyamus niger was cultivated in four different culture systems; a flask, a
stirred tank reactor, a mist bioreactor, and a unified mist bioreactor. The scopolamine …

Comparative Investigations of the Glucosinolate–Myrosinase System in Arabidopsis Suspension Cells and Hypocotyls

S Alvarez, Y He, S Chen - Plant and cell physiology, 2008 - academic.oup.com
Glucosinolates are secondary metabolites derived from amino acids. Upon hydrolysis by
myrosinases, they produce a variety of biologically active compounds. In this study, the …

Growth patterns and alkaloid accumulation in hairy root and untransformed root cultures of Datura stramonium

AM Baíza, A Quiroz, JA Ruíz… - Plant cell, tissue and …, 1998 - Springer
The aim of this work was to study the possible relationship between alkaloid production and
growth measured as: biomass increase and cellular division frequency, in Datura …

Establishment and characterization of photosynthetic hairy root cultures of Datura stramonium

IE Maldonado-Mendoza, VM Loyola-Vargas - Plant cell, Tissue and organ …, 1995 - Springer
Twelve different lines of Datura stramonium (normal and hairy) root cultures were subjected
to conditions which induce photoautotrophy. Two of the hairy root lines responded to …

Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.

A Moussous, C Paris, M Khelifi-Slaoui… - In Vitro Cellular & …, 2018 - Springer
Transgenic hairy roots of Datura spp., established using strain A4 of Agrobacterium
rhizogenes, are genetically stable and produce high levels of tropane alkaloids. To increase …

Genetic transformation of medicinal plants

YPS Bajaj, K Ishimaru - Transgenic medicinal plants, 1999 - Springer
In spite of the great progress and developments in the synthesis of chemical compounds,
plants still remain the main source of medicinal compounds flavors, dyes, pesticides, and …

Tropane alkaloid production by shoot culture of Duboisia myoporoides R. Br.

N Khanam, C Khoo, R Close, AG Khan - Phytochemistry, 2001 - Elsevier
This work demonstrates the presence of hyoscyamine and scopolamine at different stages of
shoot regeneration from non-organogenic and organogenic calli. The 11-week-old non …