A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century

E Bremer, A Calteau, A Danchin… - Microbial …, 2023 - Wiley Online Library
The vast majority of genomic sequences are automatically annotated using various software
programs. The accuracy of these annotations depends heavily on the very few manual …

The facts and family secrets of plasmids that replicate via the rolling-circle mechanism

MP Garcillán-Barcia, R Pluta… - Microbiology and …, 2022 - Am Soc Microbiol
Plasmids are self-replicative DNA elements that are transferred between bacteria. Plasmids
encode not only antibiotic resistance genes but also adaptive genes that allow their hosts to …

Sentinel cells programmed to respond to environmental DNA including human sequences

XA Nou, CA Voigt - Nature Chemical Biology, 2024 - nature.com
Monitoring environmental DNA can track the presence of organisms, from viruses to
animals, but requires continuous sampling of transient sequences from a complex milieu …

[HTML][HTML] Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria

I Ahmed, J Hahn, A Henrickson, FT Khaja… - Nature …, 2022 - nature.com
An essential step in bacterial transformation is the uptake of DNA into the periplasm, across
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …

Visualizing dynamic competence pili and DNA capture throughout the long axis of Bacillus subtilis

JD Zuke, R Erickson, KR Hummels… - Journal of …, 2023 - Am Soc Microbiol
The first step in the process of bacterial natural transformation is DNA capture. Although long
hypothesized based on genetics and functional experiments, the pilus structure responsible …

[HTML][HTML] Contractile injection systems facilitate sporogenic differentiation of Streptomyces davawensis through the action of a phage tapemeasure protein-related …

T Nagakubo, T Nishiyama, T Yamamoto… - Nature …, 2024 - nature.com
Contractile injection systems (CISs) are prokaryotic phage tail-like nanostructures loading
effector proteins that mediate various biological processes. Although CIS functions have …

[HTML][HTML] Uptake of environmental DNA in Bacillus subtilis occurs all over the cell surface through a dynamic pilus structure

A Kilb, M Burghard-Schrod, S Holtrup… - Plos Genetics, 2023 - journals.plos.org
At the transition to stationary phase, a subpopulation of Bacillus subtilis cells can enter the
developmental state of competence, where DNA is taken up through the cell envelope, and …

Watching DNA uptake: B. subtilis joins the crowd

D Dubnau - Journal of Bacteriology, 2023 - Am Soc Microbiol
ABSTRACT JD Zuke, R. Erickson, KR Hummels, and BM Burton (J Bacteriol 205: e00156-
23, 2023, https://doi. org/10.1128/jb. 00156-23) have shown that transforming DNA is …

Direct visualization of sequence-specific DNA binding by gonococcal type IV pili

A Hughes-Games, SA Davis, DJ Hill - Microbiology, 2022 - microbiologyresearch.org
Neisseria gonorrhoeae, the causative agent of gonorrhoea, is a major burden on global
healthcare systems, with an estimated~ 80–90 million new global cases annually. This …

The RecA-directed recombination pathway of natural transformation initiates at chromosomal replication forks in the pneumococcus

CHG Johnston, R Hope, AL Soulet… - Proceedings of the …, 2023 - National Acad Sciences
Homologous recombination (HR) is a crucial mechanism of DNA strand exchange that
promotes genetic repair and diversity in all kingdoms of life. Bacterial HR is driven by the …