[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
The expanded CRISPR toolbox for constructing microbial cell factories
Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
How it all begins: bacterial factors mediating the colonization of invertebrate hosts by beneficial symbionts
Beneficial associations with bacteria are widespread across animals, spanning a range of
symbiont localizations, transmission routes, and functions. While some of these associations …
symbiont localizations, transmission routes, and functions. While some of these associations …
Next-generation microbiology: from comparative genomics to gene function
Microbiology is at a turning point in its 120-year history. Widespread next-generation
sequencing has revealed genetic complexity among bacteria that could hardly have been …
sequencing has revealed genetic complexity among bacteria that could hardly have been …
Morphological and transcriptional responses to CRISPRi knockdown of essential genes in Escherichia coli
ABSTRACT CRISPR interference (CRISPRi) has facilitated the study of essential genes in
diverse organisms using both high-throughput and targeted approaches. Despite the …
diverse organisms using both high-throughput and targeted approaches. Despite the …
Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling
J Fontana, D Sparkman-Yager, I Faulkner… - Nature …, 2024 - nature.com
Engineering metabolism to efficiently produce chemicals from multi-step pathways requires
optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas …
optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas …
Sequencing-based analysis of microbiomes
Microbiomes occupy a range of niches and, in addition to having diverse compositions, they
have varied functional roles that have an impact on agriculture, environmental sciences, and …
have varied functional roles that have an impact on agriculture, environmental sciences, and …
Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum
Y Liu, R Wang, J Liu, H Lu, H Li, Y Wang, X Ni, J Li… - Science …, 2022 - science.org
Genome-scale functional screening accelerates comprehensive assessment of gene
function in cells. Here, we have established a genome-scale loss-of-function screening …
function in cells. Here, we have established a genome-scale loss-of-function screening …
CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates
F Yan, J Wang, S Zhang, Z Lu, S Li, Z Ji, C Song… - PLoS …, 2023 - journals.plos.org
CRISPR-based genome editing technology is revolutionizing prokaryotic research, but it has
been rarely studied in bacterial plant pathogens. Here, we have developed a targeted …
been rarely studied in bacterial plant pathogens. Here, we have developed a targeted …
An efficient in vivo-inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studies
While genome-wide transposon mutagenesis screens have identified numerous essential
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …