Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level

S Veit, LC Paweletz, T Günther Pomorski - Biological Chemistry, 2023 - degruyter.com
Reconstitution of membrane proteins into liposomal membranes represents a key technique
in enabling functional analysis under well-defined conditions. In this review, we provide a …

Self-assembly and biophysical properties of archaeal lipids

A Bhattacharya - Emerging Topics in Life Sciences, 2022 - portlandpress.com
Archaea constitute one of the three fundamental domains of life. Archaea possess unique
lipids in their cell membranes which distinguish them from bacteria and eukaryotes. This …

Single vesicle fluorescence-bleaching assay for multi-parameter analysis of proteoliposomes by total internal reflection fluorescence microscopy

S Veit, LC Paweletz, SSR Bohr, AK Menon… - … Applied Materials & …, 2022 - ACS Publications
Reconstitution of membrane proteins into model membranes is an essential approach for
their functional analysis under chemically defined conditions. Established model-membrane …

Using light scattering to assess how phospholipid–protein interactions affect complex I functionality in liposomes

J Eisermann, JJ Wright, JDET Wilton-Ely, J Hirst… - RSC Chemical …, 2023 - pubs.rsc.org
Complex I is an essential membrane protein in respiration, oxidising NADH and reducing
ubiquinone to contribute to the proton-motive force that powers ATP synthesis. Liposomes …

[PDF][PDF] Quantifying proton-induced membrane polarization in single biomimetic giant vesicles

R Tivony, M Fletcher, UF Keyser - Biophysical Journal, 2022 - cell.com
Proton gradients are utilized by cells to power the transport activity of many membrane
proteins. Synthetic cells, such as proteo-giant unilamellar vesicles, offer an advanced …

Quantification of membrane transport rates using optofluidics

M Fletcher - 2023 - repository.cam.ac.uk
The transport of ions and small molecules across membranes is a ubiquitous process in
biological systems. Effective studies of membrane transport phenomena require suitable …