Recombinant protein vaccines, a proven approach against coronavirus pandemics

J Pollet, WH Chen, U Strych - Advanced drug delivery reviews, 2021 - Elsevier
With the COVID-19 pandemic now ongoing for close to a year, people all over the world are
still waiting for a vaccine to become available. The initial focus of accelerated global …

Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing

R O'Flaherty, A Bergin, E Flampouri, LM Mota… - Biotechnology …, 2020 - Elsevier
The manufacturing of recombinant protein is traditionally undertaken in mammalian cell
culture. Today, speed, cost and safety are the primary considerations for process …

Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives

J Dumont, D Euwart, B Mei, S Estes… - Critical reviews in …, 2016 - Taylor & Francis
Biotherapeutic proteins represent a mainstay of treatment for a multitude of conditions, for
example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers …

[HTML][HTML] Therapeutic glycoprotein production in mammalian cells

ME Lalonde, Y Durocher - Journal of biotechnology, 2017 - Elsevier
Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

[HTML][HTML] Site-specific N-glycosylation characterization of recombinant SARS-CoV-2 spike proteins

Y Zhang, W Zhao, Y Mao, Y Chen, S Wang… - Molecular & Cellular …, 2021 - ASBMB
The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus
(SARS-CoV-2) is a determinant for viral invasion and host immune response. Herein, we …

[HTML][HTML] What does plant-based vaccine technology offer to the fight against COVID-19?

S Rosales-Mendoza, VA Márquez-Escobar… - Vaccines, 2020 - mdpi.com
The emergence of new pathogenic viral strains is a constant threat to global health, with the
new coronavirus strain COVID-19 as the latest example. COVID-19, caused by the SARS …

Structural basis for the docking of mTORC1 on the lysosomal surface

KB Rogala, X Gu, JF Kedir, M Abu-Remaileh… - Science, 2019 - science.org
The mTORC1 (mechanistic target of rapamycin complex 1) protein kinase regulates growth
in response to nutrients and growth factors. Nutrients promote its translocation to the …

Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation

D Ghaderi, M Zhang, N Hurtado-Ziola… - Biotechnology and …, 2012 - Taylor & Francis
One of the fastest growing fields in the pharmaceutical industry is the market for therapeutic
glycoproteins. Today, these molecules play a major role in the treatment of various diseases …

[图书][B] Gene cloning and DNA analysis: an introduction

TA Brown - 2020 - books.google.com
Known worldwide as the standard introductory text to this important and exciting area of
study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition …

Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus

J Stevens, O Blixt, TM Tumpey, JK Taubenberger… - science, 2006 - science.org
The hemagglutinin (HA) structure at 2.9 angstrom resolution, from a highly pathogenic
Vietnamese H5N1 influenza virus, is more related to the 1918 and other human H1 HAs …