PCR inhibitors–occurrence, properties and removal

C Schrader, A Schielke, L Ellerbroek… - Journal of applied …, 2012 - academic.oup.com
The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

[HTML][HTML] Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds

LR Harper, AS Buxton, HC Rees, K Bruce, R Brys… - Hydrobiologia, 2019 - Springer
Environmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity
monitoring tool with enormous potential to inform aquatic conservation and management …

Direct PCR amplification of forensic touch and other challenging DNA samples: a review

SE Cavanaugh, AS Bathrick - Forensic science international: Genetics, 2018 - Elsevier
DNA evidence sample processing typically involves DNA extraction, quantification, and STR
amplification; however, DNA loss can occur at both the DNA extraction and quantification …

Extraction of DNA by magnetic ionic liquids: tunable solvents for rapid and selective DNA analysis

KD Clark, O Nacham, H Yu, T Li, MM Yamsek… - Analytical …, 2015 - ACS Publications
DNA extraction represents a significant bottleneck in nucleic acid analysis. In this study,
hydrophobic magnetic ionic liquids (MILs) were synthesized and employed as solvents for …

Large scale DNA identification: The ICMP experience

TJ Parsons, RML Huel, Z Bajunović, A Rizvić - Forensic science …, 2019 - Elsevier
Abstract The International Commission on Missing Persons (ICMP) is a treaty-based
international organization with a global mandate to address the issue of missing persons. It …

[图书][B] Forensic biology: identification and DNA analysis of biological evidence

R Li - 2008 - taylorfrancis.com
Designed as an accessible introduction to basic scientific principles and their application in
professional practice, Forensic Biology provides a concise overview of the field. Focusing …

[HTML][HTML] Coming-of-age characterization of soil viruses: a user's guide to virus isolation, detection within metagenomes, and viromics

G Trubl, P Hyman, S Roux, ST Abedon - Soil Systems, 2020 - mdpi.com
The study of soil viruses, though not new, has languished relative to the study of marine
viruses. This is particularly due to challenges associated with separating virions from …

DNA/RNA preparation for molecular detection

SA Thatcher - Clinical chemistry, 2015 - academic.oup.com
BACKGROUND Effective upstream preparation of nucleic acid (NA) is important for
molecular techniques that detect unique DNA or RNA sequences. The isolated NA should …

Multiplex PCR in species authentication: probability and prospects—a review

ME Ali, MA Razzak, SBA Hamid - Food Analytical Methods, 2014 - Springer
Food forgery is one of the most articulated socio-economic concerns which contributed to
increase people's awareness on what they eat and how and where it is produced …

Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplification

J Chen, Y Xu, H Yan, Y Zhu, L Wang, Y Zhang, Y Lu… - Lab on a Chip, 2018 - pubs.rsc.org
Bacterial infections may cause severe diseases such as tuberculosis, sepsis, nephritis and
cystitis. The rapid and sensitive detection of bacteria is a prerequisite for the treatment of …