[HTML][HTML] Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear-transferred embryo development in the intraooplasmic karyoplast …

M Samiec, M Skrzyszowska - Czech Journal of …, 2005 - cjas.agriculturejournals.cz
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the
most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei …

Trichostatin A‐Mediated Epigenetic Transformation of Adult Bone Marrow‐Derived Mesenchymal Stem Cells Biases the In Vitro Developmental Capability, Quality …

M Samiec, J Opiela, D Lipiński… - BioMed research …, 2015 - Wiley Online Library
The current research was conducted to explore the in vitro developmental outcome and
cytological/molecular quality of porcine nuclear‐transferred (NT) embryos reconstituted with …

[PDF][PDF] High developmental capability of porcine cloned embryos following trichostatin A-dependent epigenomic transformation during in vitro maturation of oocytes pre-exposed …

M Samiec, M Skrzyszowska - 2012 - igbzpan.pl
The study was carried out in order to evolve, adopt and optimize the new system for
preparation of nuclear recipient cells at different stages preceding the somatic cell nuclear …

[PDF][PDF] The effect of mitochondrial genome on architectural remodeling and epigenetic reprogramming of donor cell nuclei in mammalian nuclear transfer-derived …

M Samiec - Journal of Animal and Feed Sciences, 2005 - pdfs.semanticscholar.org
There are some species-specific epigenetic factors present in the oocyte cytoplasm that may
contribute to nucleo-cytoplasmic incompatibilities either immediately after nuclear transfer …

Assessment of in vitro Developmental Capacity of Porcine Nuclear-Transferred Embryos Reconstituted with Cumulus Oophorus Cells Undergoing Vital Diagnostics for …

M Samiec, M Skrzyszowska - Annals of Animal Science, 2013 - sciendo.com
Abstract the objective of the current investigation was to extensively compare the in vitro
developmental capabilities between cloned pig embryos reconstructed with the cell nuclei of …

Creation of cloned pig embryos using contact-inhibited or serum-starved fibroblast cells analysed intravitam for apoptosis occurrence/Uzyskiwanie klonalnych …

M Samiec, M Skrzyszowska, J Opiela - Annals of Animal Science, 2013 - sciendo.com
Somatic cell cloning efficiency is determined by many factors. One of the most important
factors is the structure-functional quality of nuclear donor cells. Morphologic criteria that …

The role of mitochondrial genome (mtDNA) in somatic and embryo cloning of mammals. A review

M Samiec - J Anim Feed Sci, 2005 - jafs.com.pl
Mitochondria are semiautonomous organelles that contain double-stranded (α-helix) circular
DNA molecules (mtDNAs) of about 16300 to 16500 base pairs (bp). The mtDNA encodes …

CRISPR/Cas9-mediated biallelic knockout of IRX3 reduces the production and survival of somatic cell-cloned Bama minipigs

X Zhu, Y Wei, Q Zhan, A Yan, J Feng, L Liu, D Tang - Animals, 2020 - mdpi.com
Simple Summary Bama minipigs are a local pig breed that is unique to China and possess
several negative features, including high fat content, low feed utilization rate, and slow …

[PDF][PDF] Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos …

M Samiec, M Skrzyszowska… - Polish Journal of Veterinary …, 2012 - journals.pan.pl
The completely new strategy of pseudophysiological transcomplementary (transcytoplasmic)
activation (PP-TCA) of nuclear-transferred oocytes, which had been derived from pWAPhGH …

The possibilities of practical application of transgenic mammalian species generated by somatic cell cloning in pharmacology, veterinary medicine and …

M Samiec, M Skrzyszowska - Polish Journal of Veterinary Sciences, 2011 - agro.icm.edu.pl
Cloning of genetically-modified mammals to produce: 1) novel animal bioreactors
expressing human genes in rens, urinary bladder and the male accessory sex glands, as …