Covalent inhibitors of glycosidases and their applications in biochemistry and biology

BP Rempel, SG Withers - Glycobiology, 2008 - academic.oup.com
Glycoside hydrolases are important enzymes in a number of essential biological processes.
Irreversible inhibitors of this class of enzyme have attracted interest as probes of both …

Mechanism-based inhibitors of glycosidases: design and applications

WW Kallemeijn, MD Witte, T Wennekes… - … in carbohydrate chemistry …, 2014 - Elsevier
This article covers recent developments in the design and application of activity-based
probes (ABPs) for glycosidases, with emphasis on the different enzymes involved in …

Suite of activity-based probes for cellulose-degrading enzymes

LM Chauvigné-Hines, LN Anderson… - Journal of the …, 2012 - ACS Publications
Microbial glycoside hydrolases play a dominant role in the biochemical conversion of
cellulosic biomass to high-value biofuels. Anaerobic cellulolytic bacteria are capable of …

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

C De Boer, NGS McGregor, E Peterse… - RSC Chemical …, 2020 - pubs.rsc.org
Cellulases and related β-1, 4-glucanases are essential components of lignocellulose-
degrading enzyme mixtures. The detection of β-1, 4-glucanase activity typically relies on …

Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482

WW Wakarchuk, D Brochu, S Foote, A Robotham… - PLoS …, 2016 - journals.plos.org
The bacteria in the genus Cellulomonas are known for their ability to degrade plant cell wall
biomass. Cellulomonas fimi ATCC 484 and C. flavigena ATCC 482 have been the subject of …

Activity‐Based Profiling of Retaining β‐Glucosidases: A Comparative Study

MD Witte, MTC Walvoort, KY Li, WW Kallemeijn… - …, 2011 - Wiley Online Library
Activity‐based protein profiling (ABPP) is a versatile strategy to report on enzyme activity in
vitro, in situ, and in vivo. The development and use of ABPP tools and techniques has met …

Fluorescently labelled glycans and their applications

H Yan, RS Yalagala, F Yan - Glycoconjugate journal, 2015 - Springer
This review summarises the literature on the synthesis and applications of fluorescently
labelled carbohydrates. Due to the sensitivity of fluorescent detection, this approach …

Irreversible inhibitors and activity-based probes as research tools in chemical glycobiology

MD Witte, GA van der Marel, JMFG Aerts… - Organic & …, 2011 - pubs.rsc.org
In this review, we will discuss the enzymes that are involved in the synthesis and
degradation of glycoconjugates and we will give an overview of the inhibitors and activity …

On the reactivity and selectivity of donor glycosides in glycochemistry and glycobiology: trapped covalent intermediates

MTC Walvoort, GA van der Marel, HS Overkleeft… - Chemical …, 2013 - pubs.rsc.org
The reactivity of sugar donors and the stability of covalent intermediates formed in both
chemical and biological systems is an active subject of study in both glycochemistry and …

Activity-based proteomics probes for carbohydrate-processing enzymes: current trends and future outlook

KA Stubbs - Carbohydrate research, 2014 - Elsevier
Carbohydrate-processing enzymes are gaining more attention due to their roles in health
and disease as these enzymes are involved in the construction and deconstruction of vast …