Engineering Escherichia coli MG1655 to Efficiently Produce 3-Deacyl-4′-monophosphoryl Lipid A

Z Wang, A Zhao, J Qiao, J Yu, F He, Y Bi… - Journal of Agricultural …, 2023 - ACS Publications
Monophosphoryl lipid A, derived from Salmonella minnesota R595, has been used in
various adjuvant formulations. Escherichia coli can produce lipid A, but its structure is …

The molecular mechanism of species-specific recognition of lipopolysaccharides by the MD-2/TLR4 receptor complex

A Oblak, R Jerala - Molecular immunology, 2015 - Elsevier
Lipid A, a component of bacterial lipopolysaccharide, is a conserved microbe-associated
molecular pattern that activates the MD-2/TLR4 receptor complex. Nevertheless, bacteria …

[HTML][HTML] Remodelling of the Gram-negative bacterial Kdo2-lipid A and its functional implications

MA Valvano - Microbiology, 2022 - microbiologyresearch.org
The lipopolysaccharide (LPS) is a characteristic molecule of the outer leaflet of the Gram-
negative bacterial outer membrane, which consists of lipid A, core oligosaccharide, and O …

Genetic engineering of microorganisms for biodiesel production

H Lin, Q Wang, Q Shen, J Zhan, Y Zhao - Bioengineered, 2013 - Taylor & Francis
Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based
diesel fuel and is usually made from triacylglycerides by transesterification with alcohols …

[HTML][HTML] Construction of monophosphoryl lipid A producing Escherichia coli mutants and comparison of immuno-stimulatory activities of their lipopolysaccharides

Y Han, Y Li, J Chen, Y Tan, F Guan, X Wang - Marine Drugs, 2013 - mdpi.com
The lipid A moiety of Escherichia coli lipopolysaccharide is a hexaacylated disaccharide of
glucosamine phosphorylated at the 1-and 4′-positions. It can be recognized by the …

The impact of lipid A modification on biofilm and related pathophysiological phenotypes, endotoxicity, immunogenicity, and protection of Salmonella Typhimurium

C Hewawaduge, A Senevirathne, C Sivasankar… - Veterinary …, 2023 - Elsevier
This study presents the engineering of a less endotoxic Salmonella Typhimurium strain by
manipulating the lipid-A structure of the lipopolysaccharide (LPS) component. Salmonella …

In Vitro Assembly of the Outer Core of the Lipopolysaccharide from Escherichia coli K-12 and Salmonella typhimurium

J Qian, TA Garrett, CRH Raetz - Biochemistry, 2014 - ACS Publications
There are five distinct core structures in the lipopolysaccharides of Escherichia coli and at
least two in Salmonella isolates, which vary principally in the outer core oligosaccharide. Six …

An alternative route for UDP-diacylglucosamine hydrolysis in bacterial lipid A biosynthesis

LE Metzger IV, CRH Raetz - Biochemistry, 2010 - ACS Publications
The outer leaflet of the outer membranes of Gram-negative bacteria is composed primarily of
lipid A, the hydrophobic anchor of lipopolysaccharide. Like Escherichia coli, most Gram …

[HTML][HTML] Lysophospholipid flipping across the Escherichia coli inner membrane catalyzed by a transporter (LplT) belonging to the major facilitator superfamily

EM Harvat, YM Zhang, CV Tran, Z Zhang… - Journal of Biological …, 2005 - ASBMB
The transfer of phospholipids across membrane bilayers is protein-mediated, and most of
the established transporters catalyze the energy-dependent efflux of phospholipids from …

Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface

CM Stead, J Zhao, CRH Raetz… - Molecular …, 2010 - Wiley Online Library
Helicobacter pylori produces a unique surface lipopolysaccharide (LPS) characterized by
strikingly low endotoxicity that is thought to aid the organism in evading the host immune …