The ultimate qPCR experiment: producing publication quality, reproducible data the first time

SC Taylor, K Nadeau, M Abbasi, C Lachance… - Trends in …, 2019 - cell.com
Quantitative PCR (qPCR) is one of the most common techniques for quantification of nucleic
acid molecules in biological and environmental samples. Although the methodology is …

Essential role of KLF5 transcription factor in cell proliferation and differentiation and its implications for human diseases

JT Dong, C Chen - Cellular and molecular life sciences, 2009 - Springer
Abstract KLF5 (Kruppel-like factor 5) is a basic transcription factor binding to GC boxes at a
number of gene promoters and regulating their transcription. KLF5 is expressed during …

Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus development in cattle

N Forde, F Carter, T Fair, MA Crowe… - Biology of …, 2009 - academic.oup.com
The postovulatory rise in circulating progesterone (P4) concentrations is associated with
increased pregnancy success in beef and dairy cattle. Our study objective was to determine …

The endometrium responds differently to cloned versus fertilized embryos

S Bauersachs, SE Ulbrich… - Proceedings of the …, 2009 - National Acad Sciences
Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than in any
other species tested so far, there is a high rate of pregnancy failure that has been linked to …

Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation

N Mansouri-Attia, J Aubert, P Reinaud… - Physiological …, 2009 - journals.physiology.org
At implantation the endometrium undergoes modifications necessary for its physical
interactions with the trophoblast as well as the development of the conceptus. We aim to …

Modulation of the maternal immune system by the pre-implantation embryo

CG Walker, S Meier, MD Littlejohn, K Lehnert… - BMC genomics, 2010 - Springer
Background A large proportion of pregnancy losses occur during the pre-implantation
period, when the developing embryo is elongating rapidly and signalling its presence to the …

Triennial Reproduction Symposium: deficiencies in the uterine environment and failure to support embryonic development

GA Bridges, ML Day, TW Geary… - Journal of animal …, 2013 - academic.oup.com
Pregnancy failure in livestock can result from failure to fertilize the oocyte or embryonic loss
during gestation. The focus of this review is on cattle and factors affecting and mechanisms …

Effects of low progesterone on the endometrial transcriptome in cattle

N Forde, JP Mehta, M Minten, MA Crowe… - Biology of …, 2012 - academic.oup.com
The objective of the present study was to determine how low progesterone (P4) affects the
endometrial transcriptome, with specific emphasis on those changes that may impact …

Proteomes of the female genital tract during the oestrous cycle

C Soleilhavoup, C Riou, G Tsikis, V Labas… - Molecular & Cellular …, 2016 - ASBMB
The female genital tract includes several anatomical regions whose luminal fluids
successively interact with gametes and embryos and are involved in the fertilisation and …

Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle

P Lonergan, N Forde - animal, 2014 - cambridge.org
Early embryo development following fertilization occurs in the oviduct. However, despite
being the site of fertilization in cattle, it is possible to by-pass the oviduct by producing …