RNA‐Seq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
Deep sequencing has been revolutionizing biology and medicine in recent years, providing
single base‐level precision for our understanding of nucleic acid sequences in high …

[HTML][HTML] Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing

L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …

[HTML][HTML] Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis

JL Weirather, M de Cesare, Y Wang, P Piazza… - …, 2017 - ncbi.nlm.nih.gov
Background: Given the demonstrated utility of Third Generation Sequencing [Pacific
Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many …

[HTML][HTML] Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza

Y Ma, G Cui, T Chen, X Ma, R Wang, B Jin… - Nature …, 2021 - nature.com
Tanshinones are the bioactive nor-diterpenoid constituents of the Chinese medicinal herb
Danshen (Salvia miltiorrhiza). These groups of chemicals have the characteristic furan D …

[HTML][HTML] A survey of the sorghum transcriptome using single-molecule long reads

SE Abdel-Ghany, M Hamilton, JL Jacobi… - Nature …, 2016 - nature.com
Alternative splicing and alternative polyadenylation (APA) of pre-mRNAs greatly contribute
to transcriptome diversity, coding capacity of a genome and gene regulatory mechanisms in …

[HTML][HTML] Analysis of the genome sequence of the medicinal plant Salvia miltiorrhiza

H Xu, J Song, H Luo, Y Zhang, Q Li, Y Zhu, J Xu, Y Li… - Molecular plant, 2016 - cell.com
Salvia miltiorrhiza Bunge (Danshen) is a medicinal plant of the Lamiaceae family, and its
dried roots have long been used in traditional Chinese medicine with hydrophilic phenolic …

[HTML][HTML] Tanshinone and salvianolic acid biosynthesis are regulated by SmMYB98 in Salvia miltiorrhiza hairy roots

X Hao, Z Pu, G Cao, D You, Y Zhou, C Deng… - Journal of Advanced …, 2020 - Elsevier
Salvia miltiorrhiza Bunge is an herb rich in bioactive tanshinone and salvianolic acid
compounds. It is primarily used as an effective medicine for treating cardiovascular and …

[HTML][HTML] Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza

B Li, G Cui, G Shen, Z Zhan, L Huang, J Chen, X Qi - Scientific reports, 2017 - nature.com
CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model
plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report …

Comprehensive profiling of rhizome‐associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis)

T Wang, H Wang, D Cai, Y Gao, H Zhang… - The Plant …, 2017 - Wiley Online Library
Moso bamboo (Phyllostachys edulis) represents one of the fastest‐spreading plants in the
world, due in part to its well‐developed rhizome system. However, the post‐transcriptional …

CRISPR/Cas9-mediated efficient targeted mutagenesis of RAS in Salvia miltiorrhiza

Z Zhou, H Tan, Q Li, J Chen, S Gao, Y Wang, W Chen… - Phytochemistry, 2018 - Elsevier
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
(CRISPR-associated) system is a powerful genome editing tool that has been used in many …