Oocyte-specific deletion of complex and hybrid N-glycans leads to defects in preovulatory follicle and cumulus mass development

SA Williams, P Stanley - Reproduction, 2009 - rep.bioscientifica.com
An ovarian follicle is composed of an oocyte and surrounding theca and granulosa cells.
Oocytes are stored in an arrested state within primordial follicles until they are signaled to re …

Improving native human sperm freezing protection by using a modified vitrification method

D Zhou, XM Wang, RX Li, YZ Wang… - Asian Journal of …, 2021 - journals.lww.com
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa
in clinical practice. However, it has been shown to have a negative impact on sperm function …

A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm

S Stigliani, A Amaro, F Reggiani, E Maccarini… - International Journal of …, 2024 - mdpi.com
Sperm cryopreservation is a procedure widely used to store gametes for later use, to
preserve fertility in patients prior to gonadotoxic treatments or surgery, and for sperm …

Loss of heat shock protein 70 from apical region of buffalo (Bubalus bubalis) sperm head after freezing and thawing

T Varghese, BC Divyashree, SC Roy, KS Roy - Theriogenology, 2016 - Elsevier
The post-thaw fertility of frozen-thawed mammalian spermatozoa is substantially low as
compared with that of fresh sperm. Furthermore, the post-thaw fertility of the cryopreserved …

Effects of vasectomy on gene expression profiling along the human epididymis

V Thimon, E Calvo, O Koukoui, C Légaré… - Biology of …, 2008 - academic.oup.com
Worldwide, almost 100 million men rely on vasectomy for male contraceptive purposes. Due
to changes in their personal lives, an increasing number of these men request surgical …

Pretreatment with cholesterol-loaded cyclodextrins prevents loss of motility associated proteins during cryopreservation of addra gazelle (Nanger dama ruficollis) …

J Wojtusik, Y Wang, BS Pukazhenthi - Cryobiology, 2018 - Elsevier
Sperm cryopreservation is challenging, often resulting in irreversible damage to
spermatozoa, as indicated by decreased motility, viability, and/or acrosomal integrity …

Sperm motility evaluation following long‐term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

A Fabbrocini, R D'Adamo, S Pelosi… - Journal of Applied …, 2015 - Wiley Online Library
The aim of this study was to evaluate, by computer‐assisted analysis, the motility parameters
of cryopreserved sea bream spermatozoa after prolonged storage (up to 5 years) in liquid …

PERSPECTIVE: The comet assay as a method for assessing DNA damage in cryopreserved samples

BP Plitta-Michalak, A Ramos, D Stępień, M Trusiak… - …, 2024 - ingentaconnect.com
The preservation of the nuclear genome's integrity is paramount for the viability and overall
health of cells, tissues, and organisms. DNA, being susceptible to damage under …

Sole use of sucrose in human sperm cryopreservation

AM Hossain, CO Osuamkpe - Archives of Andrology, 2007 - Taylor & Francis
Glycerol alone or in combination with other additives is one of the most widely used and
successful cryoprotectants for human sperm. The glycerol method requires rigorous post …

Stress and damage mechanisms in Dendrobium nobile Lindl. protocorm-like bodies during pre- and post-liquid nitrogen exposure in cryopreservation revealed by …

W Di, X Jiang, J Xu, M Jia, B Li, Y Liu - In Vitro Cellular & Developmental …, 2018 - Springer
Liquid nitrogen (LN) exposure, a crucial step in cryopreservation, is mainly responsible for
the reduced viability of protocorm-like bodies (PLBs) in Dendrobium nobile Lindl. To …