Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

From angstroms to nanometers: measuring interatomic distances by solid-state NMR

AA Shcherbakov, J Medeiros-Silva, N Tran… - Chemical …, 2021 - ACS Publications
Internuclear distances represent one of the main structural constraints in molecular structure
determination using solid-state NMR spectroscopy, complementing chemical shifts and …

Highly bioresistant, hydrophilic and rigidly linked trityl-nitroxide biradicals for cellular high-field dynamic nuclear polarization

R Yao, D Beriashvili, W Zhang, S Li, A Safeer… - Chemical …, 2022 - pubs.rsc.org
Cellular dynamic nuclear polarization (DNP) has been an effective means of overcoming the
intrinsic sensitivity limitations of solid-state nuclear magnetic resonance (ssNMR) …

[HTML][HTML] Understanding molecular mechanisms of biologics drug delivery and stability from NMR spectroscopy

P Phyo, X Zhao, AC Templeton, W Xu… - Advanced Drug Delivery …, 2021 - Elsevier
Protein therapeutics carry inherent limitations of membrane impermeability and structural
instability, despite their predominant role in the modern pharmaceutical market. Effective …

SARS-CoV-2 envelope protein forms clustered pentamers in lipid bilayers

NH Somberg, WW Wu, J Medeiros-Silva, AJ Dregni… - Biochemistry, 2022 - ACS Publications
The SARS-CoV-2 envelope (E) protein is a viroporin associated with the acute respiratory
symptoms of COVID-19. E forms cation-selective ion channels that assemble in the lipid …

Direct observation of cholesterol dimers and tetramers in lipid bilayers

MR Elkins, A Bandara, GA Pantelopulos… - The Journal of …, 2021 - ACS Publications
Cholesterol is a ubiquitous component of mammalian cell membranes and affects
membrane protein function. Although cholesterol-mediated formation of ordered membrane …

Protein amphipathic helix insertion: A mechanism to induce membrane fission

MA Zhukovsky, A Filograna, A Luini, D Corda… - Frontiers in cell and …, 2019 - frontiersin.org
One of the fundamental features of biomembranes is the ability to fuse or to separate. These
processes called respectively membrane fusion and fission are central in the homeostasis of …

Characterization of dielectric properties and their impact on MAS-DNP NMR applications

FJ Scott, T Dubroca, RW Schurko, S Hill… - Journal of Magnetic …, 2024 - Elsevier
The dielectric properties of materials play a crucial role in the propagation and absorption of
microwave beams employed in Magic Angle Spinning− Dynamic Nuclear Polarization (MAS …

Substitution-type luminescent MOF sensor with built-in capturer for selective cholesterol detection in blood serum

M Gong, J Yang, Y Li, Q Zhuang, J Gu - Journal of Materials Chemistry …, 2019 - pubs.rsc.org
The development of an effective strategy for the accurate detection of cholesterol in human
serum is of great significance. Herein, a novel luminescent metal–organic framework …