Chinese hamster ovary (CHO) cells are among the most widely used mammalian cell lines
in the biopharmaceutical industry. Therefore, it is not surprising that significant efforts have …

Recent advances in CRISPR-Cas genome editing technology have been instrumental in
improving the efficiency to produce genetically modified animal models. In this study we …

Simple Summary Approaches to manipulate the genome of an organism, both selectively
and accurately, are powerful techniques that can influence research practice, with extensive …

Introduction: Hemophilia A (HA) is the most common genetic bleeding disorder caused by
mutations in the F8 gene encoding coagulation factor VIII (FVIII). As the second predominant …

Recombinant Chinese hamster ovary (CHO) cell line development for complex
biotherapeutic production is conventionally based on the random integration (RI) approach …

Transcriptomic responses to neurotransmitters contribute to the complex processes driving
memory and addiction. Advances in both measurement methods and experimental models …

Precise insertion of fluorescent proteins into lineage-specific genes in human pluripotent
stem cells (hPSCs) presents challenges due to low knockin efficiency and difficulties in …

Genome editing technology is widely used to produce genetically modified animals,
including rats. Cytoplasmic or pronuclear injection of DNA repair templates and CRISPR …

Targeted knock-in supported by the CRISPR/Cas systems enables the insertion, deletion,
and substitution of genome sequences exactly as designed. Although this technology is …

IgM is the major circulating Ig isotype in teleost fish, showing in Antarctic fish unique features
such as an extraordinary long hinge region, which plays a crucial role in antibody structure …