DNA base editors use deaminases fused to a programmable DNA-binding protein for
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …

Current DNA base editors contain nuclease and DNA deaminase that enables deamination
of cytosine (C) or adenine (A), but no method for guanine (G) or thymine (T) editing is …

Although single-nucleotide variants (SNVs) make up the majority of cancer-associated
genetic changes and have been comprehensively catalogued, little is known about their …

Over recent years, zinc-dependent deaminases have attracted increasing interest as key
components of nucleic acid editing tools that can generate point mutations at specific sites in …

Genome editing technologies generate targeted DNA lesions and rely on cellular DNA
repair pathways for resolution. Understanding the DNA repair mechanisms responsible for …

Prion disease is caused by misfolding of the prion protein (PrP) into pathogenic self-
propagating conformations, leading to rapid-onset dementia and death. However …

CRISPR nucleases generate a broad spectrum of mutations that includes undesired editing
outcomes. Here, we develop optimized C-to-T base editing systems for the generation of …

DNA base editors enable direct editing of adenine (A), cytosine (C), or guanine (G), but there
is no base editor for direct thymine (T) editing currently. Here we develop two deaminase …

Cas9 protein without sgRNAs can induce genomic damage at the cellular level in vitro.
However, whether the detrimental effects occur in embryos after Cas9 treatment remains …

Cancers arise through acquisition of mutations in genes that regulate core biological
processes like cell proliferation and cell death. Decades of cancer research have led to the …