[HTML][HTML] High variability of genomic instability and gene expression profiling in different HeLa clones
The HeLa cell line is one of the most popular cell lines in biomedical research, despite its
well-known chromosomal instability. We compared the genomic and transcriptomic profiles …
well-known chromosomal instability. We compared the genomic and transcriptomic profiles …
qDRIP: a method to quantitatively assess RNA–DNA hybrid formation genome-wide
R-loops are dynamic, co-transcriptional nucleic acid structures that facilitate physiological
processes but can also cause DNA damage in certain contexts. Perturbations of …
processes but can also cause DNA damage in certain contexts. Perturbations of …
H3K36me3-mediated mismatch repair preferentially protects actively transcribed genes from mutation
Histone H3 trimethylation at lysine 36 (H3K36me3) is an important histone mark involved in
both transcription elongation and DNA mismatch repair (MMR). It is known that H3K36me3 …
both transcription elongation and DNA mismatch repair (MMR). It is known that H3K36me3 …
PRC 1‐labeled microtubule bundles and kinetochore pairs show one‐to‐one association in metaphase
In the mitotic spindle, kinetochore microtubules form k‐fibers, whereas overlap or interpolar
microtubules form antiparallel arrays containing the cross‐linker protein regulator of …
microtubules form antiparallel arrays containing the cross‐linker protein regulator of …
Guidelines for optimized gene knockout using CRISPR/Cas9
CV Campenhout, P Cabochette, AC Veillard… - …, 2019 - Taylor & Francis
CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic
models both for fundamental and preclinical research. Despite its apparent simplicity, the …
models both for fundamental and preclinical research. Despite its apparent simplicity, the …
Engineering and evaluating drug delivery particles in microfluidic devices
The development of new and improved particle-based drug delivery is underpinned by an
enhanced ability to engineer particles with high fidelity and integrity, as well as increased …
enhanced ability to engineer particles with high fidelity and integrity, as well as increased …
Systematic review: characteristics and preclinical uses of bladder cancer cell lines
T Zuiverloon, FC De Jong, JC Costello… - Bladder …, 2018 - content.iospress.com
Background: Bladder cancer (BC) cell lines are indispensable in basic and preclinical
research. Currently, an up-to-date and comprehensive overview of available BC cell lines is …
research. Currently, an up-to-date and comprehensive overview of available BC cell lines is …
The Mayo Clinic Florida microdosimetric kinetic model of clonogenic survival: formalism and first benchmark against in vitro and in silico data
A Parisi, CJ Beltran, KM Furutani - Physics in Medicine & Biology, 2022 - iopscience.iop.org
Objective. To develop a new model (Mayo Clinic Florida microdosimetric kinetic model, MCF
MKM) capable of accurately describing the in vitro clonogenic survival at low and high linear …
MKM) capable of accurately describing the in vitro clonogenic survival at low and high linear …
DNA replication acts as an error correction mechanism to maintain centromere identity by restricting CENP-A to centromeres
Chromatin assembled with the histone H3 variant CENP-A is the heritable epigenetic
determinant of human centromere identity. Using genome-wide mapping and reference …
determinant of human centromere identity. Using genome-wide mapping and reference …
Peroxin-dependent targeting of a lipid-droplet-destined membrane protein to ER subdomains
Lipid droplets (LDs) are endoplasmic reticulum (ER)-derived lipid storage organelles
uniquely encapsulated by phospholipid monolayers. LD membrane proteins are embedded …
uniquely encapsulated by phospholipid monolayers. LD membrane proteins are embedded …