Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Pseudomonas putida KT2440 strain metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and pentose …

PI Nikel, M Chavarría, T Fuhrer, U Sauer… - Journal of Biological …, 2015 - ASBMB
The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities

J Nogales, J Mueller, S Gudmundsson… - Environmental …, 2020 - Wiley Online Library
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …

Carbon catabolite repression in Pseudomonas: optimizing metabolic versatility and interactions with the environment

F Rojo - FEMS microbiology reviews, 2010 - academic.oup.com
Metabolically versatile free-living bacteria have global regulation systems that allow cells to
selectively assimilate a preferred compound among a mixture of several potential carbon …

Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity

CW Johnson, D Salvachúa, P Khanna, H Smith… - Metabolic engineering …, 2016 - Elsevier
The conversion of biomass-derived sugars and aromatic molecules to cis, cis-muconic acid
(referred to hereafter as muconic acid or muconate) has been of recent interest owing to its …

From dirt to industrial applications: Pseudomonas putida as a synthetic biology chassis for hosting harsh biochemical reactions

PI Nikel, M Chavarria, A Danchin… - Current opinion in chemical …, 2016 - Elsevier
Highlights•SynBio needs chassis with superior genetic, biochemical, and physiological
traits.•Environmental bacteria are endowed with many advantageous metabolic properties.• …

Industrial biotechnology of Pseudomonas putida and related species

I Poblete-Castro, J Becker, K Dohnt… - Applied microbiology …, 2012 - Springer
Since their discovery many decades ago, Pseudomonas putida and related subspecies
have been intensively studied with regard to their potential application in industrial …