A cellular resolution atlas of Broca's area

I Costantini, L Morgan, J Yang, Y Balbastre… - Science …, 2023 - science.org
Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of
scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex …

Neurophotonic tools for microscopic measurements and manipulation: status report

AS Abdelfattah, S Ahuja, T Akkin, SR Allu… - …, 2022 - spiedigitallibrary.org
Neurophotonics was launched in 2014 coinciding with the launch of the BRAIN Initiative
focused on development of technologies for advancement of neuroscience. For the last …

[HTML][HTML] Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation

Q Zhou, Z Chen, YH Liu, M El Amki, C Glück… - Nature …, 2022 - nature.com
Wide-field fluorescence imaging is an indispensable tool for studying large-scale
biodynamics. Limited space-bandwidth product and strong light diffusion make conventional …

openFrame: A modular, sustainable, open microscopy platform with single‐shot, dual‐axis optical autofocus module providing high precision and long range of …

J Lightley, S Kumar, MQ Lim, E Garcia… - Journal of …, 2023 - Wiley Online Library
Abstract 'openFrame'is a modular, low‐cost, open‐hardware microscopy platform that can
be configured or adapted to most light microscopy techniques and is easily upgradeable or …

[HTML][HTML] A guide to perform 3D histology of biological tissues with fluorescence microscopy

A Laurino, A Franceschini, L Pesce, L Cinci… - International Journal of …, 2023 - mdpi.com
The analysis of histological alterations in all types of tissue is of primary importance in
pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and …

Robust deep learning optical autofocus system applied to automated multiwell plate single molecule localization microscopy

J Lightley, F Görlitz, S Kumar, R Kalita… - Journal of …, 2022 - Wiley Online Library
We presenta robust, long‐range optical autofocus system for microscopy utilizing machine
learning. This can be useful for experiments with long image data acquisition times that may …

Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography

N Wang, X Wang, T Yan, H Xie, L Wang, F Ren… - Science …, 2023 - science.org
Mesoscale volumetric imaging is of great importance for the study of bio-organisms. Among
others, optical projection tomography provides unprecedented structural details of …

[HTML][HTML] Virtual staining of defocused autofluorescence images of unlabeled tissue using deep neural networks

Y Zhang, L Huang, T Liu, K Cheng, K de Haan… - Intelligent …, 2022 - spj.science.org
Deep learning-based virtual staining was developed to introduce image contrast to label-
free tissue sections, digitally matching the histological staining, which is time-consuming …

[HTML][HTML] COMBINe enables automated detection and classification of neurons and astrocytes in tissue-cleared mouse brains

Y Cai, X Zhang, C Li, HT Ghashghaei… - Cell Reports Methods, 2023 - cell.com
Tissue clearing renders entire organs transparent to accelerate whole-tissue imaging; for
example, with light-sheet fluorescence microscopy. Yet, challenges remain in analyzing the …

Flexible and universal autofocus based on amplitude difference of fractional Fourier transform

Z Li, X Zhou, Y Wang, G Huang, S Liu, B Gao… - Optics and Lasers in …, 2024 - Elsevier
Autofocus technology is a crucial technique in computational optical imaging. The clarity
evaluation function (CEF) serves as the criterion for autofocus, making it the core of the …