When duplex DNA is altered in almost any way (replicated, recombined, or repaired), single
strands of DNA are usually intermediates, and single-stranded DNA binding (SSB) proteins …

▪ Abstract DNA primases are enzymes whose continual activity is required at the DNA
replication fork. They catalyze the synthesis of short RNA molecules used as primers for …

▪ Abstract The elaborate process of genomic replication requires a large collection of
proteins properly assembled at a DNA replication fork. Several decades of research on the …

We present single-molecule studies of the Escherichia coli replication machinery. We
visualize individual E. coli DNA polymerase III (Pol III) holoenzymes engaging in primer …

Replisomes are the protein assemblies that replicate DNA. They function as molecular
motors to catalyze template-mediated polymerization of nucleotides, unwinding of DNA, the …

DNA primase synthesizes short RNA primers that replicative polymerases further elongate in
order to initiate the synthesis of all new DNA strands. Thus, primase owes its existence to the …

Rescue of arrested and collapsed replication forks is essential for maintenance of genomic
integrity. One system for origin of replication-independent loading of the DnaB replicative …

DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …

The coordination of primase function within the replisome is an essential but poorly
understood feature of lagging strand synthesis. By using crystallography and small-angle X …

Cellular replication forks are powered by ring-shaped, hexameric helicases that encircle and
unwind DNA. To better understand the molecular mechanisms and control of these …