Raman microspectroscopy for microbiology

KS Lee, Z Landry, FC Pereira, M Wagner… - Nature Reviews …, 2021 - nature.com
Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to
assess the chemical composition of individual live microorganisms in near real time. In this …

Microbial storage and its implications for soil ecology

K Mason-Jones, SL Robinson, GF Veen… - The ISME …, 2022 - academic.oup.com
Organisms throughout the tree of life accumulate chemical resources, in particular forms or
compartments, to secure their availability for future use. Here we review microbial storage …

CheckM2: a rapid, scalable and accurate tool for assessing microbial genome quality using machine learning

A Chklovski, DH Parks, BJ Woodcroft, GW Tyson - Nature Methods, 2023 - nature.com
Advances in sequencing technologies and bioinformatics tools have dramatically increased
the recovery rate of microbial genomes from metagenomic data. Assessing the quality of …

[HTML][HTML] Oxford Nanopore R10. 4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short …

M Sereika, RH Kirkegaard, SM Karst, TY Michaelsen… - Nature …, 2022 - nature.com
Abstract Long-read Oxford Nanopore sequencing has democratized microbial genome
sequencing and enables the recovery of highly contiguous microbial genomes from isolates …

[HTML][HTML] MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

MKD Dueholm, M Nierychlo, KS Andersen… - Nature …, 2022 - nature.com
Microbial communities are responsible for biological wastewater treatment, but our
knowledge of their diversity and function is still poor. Here, we sequence more than 5 million …

Generating lineage-resolved, complete metagenome-assembled genomes from complex microbial communities

DM Bickhart, M Kolmogorov, E Tseng, DM Portik… - Nature …, 2022 - nature.com
Microbial communities might include distinct lineages of closely related organisms that
complicate metagenomic assembly and prevent the generation of complete metagenome …

High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome

W Zheng, S Zhao, Y Yin, H Zhang, DM Needham… - Science, 2022 - science.org
Characterizing complex microbial communities with single-cell resolution has been a long-
standing goal of microbiology. We present Microbe-seq, a high-throughput method that …

[HTML][HTML] Nanopore long-read-only metagenomics enables complete and high-quality genome reconstruction from mock and complex metagenomes

L Liu, Y Yang, Y Deng, T Zhang - Microbiome, 2022 - Springer
Background The accurate and comprehensive analyses of genome-resolved metagenomics
largely depend on the reconstruction of reference-quality (complete and high-quality) …

Active predation, phylogenetic diversity, and global prevalence of myxobacteria in wastewater treatment plants

L Zhang, X Huang, J Zhou, F Ju - The ISME journal, 2023 - academic.oup.com
The operation of modern wastewater treatment plants (WWTPs) is driven by activated sludge
microbiota, a complex assemblage of trophically interacting microorganisms. Microbial …

[HTML][HTML] Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Z Kondrotaite, LC Valk, F Petriglieri… - Frontiers in …, 2022 - frontiersin.org
The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly
abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its …