Highlights•We describe three long-read sequencing technologies and their
characteristics.•We describe available algorithms for long-read assembly and their …

The discovery and implementation of antibiotics in the early twentieth century transformed
human health and wellbeing. Chemical synthesis enabled the development of the first …

The increasing prevalence of infections due to multidrug-resistant (MDR) gram-negative
bacteria constitutes a serious threat to global public health due to the limited treatment …

Hypervirulent K. pneumoniae (hvKp) is an evolving pathotype that is more virulent than
classical K. pneumoniae (cKp). hvKp usually infects individuals from the community, who are …

Twenty years ago, the publication of the first bacterial genome sequence, from Haemophilus
influenzae, shook the world of bacteriology. In this Timeline, we review the first two decades …

Antimicrobial resistance (AMR) is now a global threat. Its emergence rests on antimicrobial
overuse in humans and food-producing animals; globalization and suboptimal infection …

Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance
knowledge and understanding of infectious diseases and clinical microbiology. The growth …

Whole-genome sequence (WGS) analysis has revolutionized the food safety industry by
enabling high-resolution typing of foodborne bacteria. Higher resolving power allows …

Carbapenem-resistant Enterobacteriaceae (CRE) are major health care-associated
pathogens and responsible for hospital outbreaks worldwide. To prevent a further increase …

The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was
commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline …