The transcription cycle can be roughly divided into three stages: initiation, elongation, and
termination. Understanding the molecular events that regulate all these stages requires a …

RNA turnover is an integral part of cellular RNA homeostasis and gene expression
regulation. Whereas the cytoplasmic control of protein-coding mRNA is often the focus of …

Analogous to alternative splicing, alternative polyadenylation (APA) has long been thought
to occur independently at proximal and distal polyA sites. Using fractionation-seq, we …

The end of the RNA polymerase II (Pol II) transcription cycle is strictly regulated to prevent
interference between neighbouring genes and to safeguard transcriptome integrity. The …

Termination is a ubiquitous phase in every transcription cycle but is incompletely understood
and a subject of debate. We used gene editing as a new approach to address its mechanism …

The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient
termination. A key player in this process is the cleavage and polyadenylation (CPA) factor …

The synthesis of nascent RNA is a discontinuous process in which phases of productive
elongation by RNA polymerase are interrupted by frequent pauses. Transcriptional pausing …

CDK7 phosphorylates the RNA polymerase II (pol II) C-terminal domain CTD and activates
the P-TEFb-associated kinase CDK9, but its regulatory roles remain obscure. Here, using …

Gene transcription by RNA polymerase II (Pol II) is under control of promoters and distal
regulatory elements known as enhancers. Enhancers are themselves transcribed by Pol II …

Elongation factor Paf1C regulates several stages of the RNA polymerase II (Pol II)
transcription cycle, although it is unclear how it modulates Pol II distribution and progression …