To be properly expressed, genes need to be accompanied by a terminator, a region
downstream of the coding sequence that contains the information necessary for the …

The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is composed of a repetition
of YSPTSPS heptads and functions as a loading platform for protein complexes that regulate …

Reversible phosphorylation of Pol II and accessory factors helps order the transcription
cycle. Here, we define two kinase-phosphatase switches that operate at different points in …

Accurate regulation of mRNA termination is required for correct gene expression. Here, we
describe a role for SCAF4 and SCAF8 as anti-terminators, suppressing the use of early …

The genomes of kinetoplastids are organized into polycistronic transcription units that are
flanked by a modified DNA base (base J, beta-D-glucosyl-hydroxymethyluracil). Previous …

Transcription elongation by RNA polymerase II (Pol II) has emerged as a regulatory hub in
gene expression. A key control point occurs during early transcription elongation when Pol II …

Complex regulation of gene expression in mammals has evolved from simpler eukaryotic
systems, yet the mechanistic features of this evolution remain elusive. Here, we compared …

The human genome encodes thousands of non-coding RNAs. Many of these terminate early
and are then rapidly degraded, but how their transcription is restricted is poorly understood …

Recent observations showed that nascent RNA polymerase II transcripts, pre-mRNAs, and
noncoding RNAs are highly susceptible to premature 3′-end cleavage and …

Targeted protein degradation, with its rapid protein depletion kinetics, allows the
measurement of acute changes in the cell. The auxin-inducible degron (AID) system, rapidly …