CRISPR-based diagnostics

MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …

[HTML][HTML] Review of COVID-19 testing and diagnostic methods

O Filchakova, D Dossym, A Ilyas, T Kuanysheva… - Talanta, 2022 - Elsevier
More than six billion tests for COVID-19 has been already performed in the world. The
testing for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and …

Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples

L Wang, X Wang, Y Wu, M Guo, C Gu, C Dai… - Nature Biomedical …, 2022 - nature.com
The detection of samples at ultralow concentrations (one to ten copies in 100 μl) in biofluids
is hampered by the orders-of-magnitude higher amounts of 'background'biomolecules. Here …

Tools and techniques for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 detection

SH Safiabadi Tali, JJ LeBlanc, Z Sadiq… - Clinical microbiology …, 2021 - Am Soc Microbiol
SUMMARY The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory disease coronavirus 2 (SARS-CoV-2), has led to millions of confirmed cases and …

Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a

S Lu, X Tong, Y Han, K Zhang, Y Zhang… - Nature biomedical …, 2022 - nature.com
CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not
fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection …

Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

NL Welch, M Zhu, C Hua, J Weller, ME Mirhashemi… - Nature medicine, 2022 - nature.com
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …

Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants

H De Puig, RA Lee, D Najjar, X Tan, LR Soenksen… - Science …, 2021 - science.org
The COVID-19 pandemic highlights the need for diagnostics that can be rapidly adapted
and deployed in a variety of settings. Several SARS-CoV-2 variants have shown worrisome …

Rapid, point‐of‐care antigen tests for diagnosis of SARS‐CoV‐2 infection

J Dinnes, P Sharma, S Berhane… - Cochrane database …, 2022 - cochranelibrary.com
Background Accurate rapid diagnostic tests for SARS‐CoV‐2 infection would be a useful
tool to help manage the COVID‐19 pandemic. Testing strategies that use rapid antigen tests …

Lateral flow test engineering and lessons learned from COVID-19

J Budd, BS Miller, NE Weckman, D Cherkaoui… - Nature Reviews …, 2023 - nature.com
The acceptability and feasibility of large-scale testing with lateral flow tests (LFTs) for clinical
and public health purposes has been demonstrated during the COVID-19 pandemic. LFTs …

Rapid electrochemical detection of coronavirus SARS-CoV-2

T Chaibun, J Puenpa, T Ngamdee… - Nature …, 2021 - nature.com
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 …