In recent years, the use of integrative, information-driven computational approaches for
modeling the structure of biomolecules has been increasing in popularity. These are now …

Site-directed scanning mutagenesis is a powerful protein engineering technique which
allows studies of protein functionality at single amino acid resolution and design of stabilized …

There are nearly 2000 mutations in the CFTR gene associated with cystic fibrosis disease,
and to date, the only approved drug, Kalydeco, has been effective in rescuing the functional …

Oxygen (O 2) is essential for the survival and reproduction of most species. However, in
recent years, with global climate change and the increasing impact of human activities on …

High-throughput assay systems have had a large impact on understanding the mechanisms
of basic cell functions. However, high-throughput assays that directly assess molecular …

The calcium-activated chloride channel ANO1 regulates multiple physiological processes.
However, little is known about the mechanism of channel gating and regulation of ANO1 …

Prostaglandins play a critical physiological role in both cardiovascular and immune systems,
acting through their interactions with 9 prostanoid G protein-coupled receptors (GPCRs) …

High throughput random mutagenesis is a powerful tool to identify which residues are
important for the function of a protein, and gain insight into its structure-function relation. The …

Over the decades, practical biotechnology researchers have aimed to improve naturally
occurring proteins and create novel ones. It is widely recognized that coupling protein …

Intrinsically disordered protein IDPs do not adopt stable three-dimensional structures but
exist as highly dynamic ensembles of conformations. Amyloid-β is an IDP that is at the center …