Gene therapy is now being applied to the treatment of a wide variety of acquired and
inherited diseases. One of the rate-limiting steps for successful gene therapy is the efficiency …

The development and widespread use of vaccines against infectious agents have been a
great triumph of medical science. One reason for the success of currently available vaccines …

Structural, biochemical and biophysical studies of eukaryotic membrane proteins are often
hampered by difficulties in overexpression of the candidate molecule. Baculovirus …

DNA vaccines were introduced less than a decade ago but have already been applied to a
wide range of infectious and malignant diseases. Here we review the current understanding …

The construction of cDNA clones encoding large-size RNA molecules of biological interest,
like coronavirus genomes, which are among the largest mature RNA molecules known to …

Coronaviruses (CoVs) infect humans and many animal species, and are associated with
respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV …

Alphaviruses are positive-strand RNA viruses that can mediate efficient cytoplasmic gene
expression in insect and vertebrate cells. Through recombinant DNA technology, the …

Recombinant nucleic acids are considered as promising next-generation vaccines. These
vaccines express the native antigen upon delivery into tissue, thus mimicking live attenuated …

Cancer vaccines targeting'self'antigens that are expressed at consistently high levels by
tumor cells are potentially useful in immunotherapy, but immunological tolerance may block …

Alphavirus expression systems based on suicidal virus particles carrying recombinant
replicons have proven to be a very efficient way to deliver genes for heterologous protein …