Implementing strategies in synthetic biology to maximize membrane protein production

MD Rieth - Authorea Preprints, 2023 - authorea.com
Membrane proteins represent a class of proteins that are difficult targets to characterize.
Their structural and functional characterization requires that they first be produced at …

Macromolecular crowding affects protein diffusion in stress fibers as revealed by fluorescence correlation spectroscopy

A Buenaventura, T Saito, T Kanao, D Matsunaga… - 2023 - researchsquare.com
The diffusion of cell components such as proteins is crucial to the function of all living cells.
The abundance of macromolecules in the cell is likely to cause a state of macromolecular …

How does an ectodomain of membrane-associated proteins stand upright and exert robust signal?

S Lankipalli, UA Ramagopal - bioRxiv, 2020 - biorxiv.org
Even after decades of research, a comprehensive mechanism that elucidates the
underpinnings of signaling through the cell membrane is still elusive. Here, we address a …

Cellular stress characterisation in S. cerevisiae budding yeast using optical microscopy

S Lecinski - 2022 - etheses.whiterose.ac.uk
The term" cellular stress" covers various environmental and metabolic events that threaten
cell survival. In response, eukaryotic cells can adapt their metabolism to acute changes in …

[引用][C] Effects of macromolecular crowding on protein targeting and translocation via SecYEG

M Löwe

[HTML][HTML] Hydrophobic mismatch and sequence specificity compete when transmembrane helix-helix interactions are measured with the TOXCAT assay

N Hellmann, D Schneider - Frontiers in Chemistry, 2022 - frontiersin.org
Genetic assays capable of measuring the propensity of transmembrane helices to
oligomerize within the cytoplasmic membrane of the bacterium E. coli are frequently used …

Enhanced therapeutic window for antimicrobial Pept-ins by investigating their structure-activity relationship

G Wu, L Khodaparast, L Khodaparast… - PloS one, 2023 - journals.plos.org
The overconsumption and inappropriate use of antibiotics is escalating antibiotic resistance
development, which is now one of the 10 top threats to global health. Introducing antibiotics …

[PDF][PDF] Single Molecule Delivery and Detection into Crowded Environment with a Solid-State Nanopore

CC Chau - 2021 - etheses.whiterose.ac.uk
Cultured cell manipulation by the intracellular delivery of exogenous biomolecules like DNA
and proteins is the cornerstone of modern experimental cell biology. The intracellular …

Protein segregation and conformation in antigen-receptor triggering: a quantitative fluorescence microscopy study

J Humphrey - 2021 - repository.cam.ac.uk
Antigen receptors play a central role in determining whether an immune response is
required. The remarkable diversity of their extracellular domains allows them to detect …

Research Advances in Plant Golgi Apparatus

T Lu, Y Gong, L Zhao, F Yan - Agricultural Biotechnology, 2021 - search.proquest.com
Golgi apparatus, together with endoplasmic reticula, vacuoles and plasma membrane,
constitutes the endoplasmic system of plant cells. It plays an important role in the secretion …