Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …

Conjugation, the process by which plasmid DNA is transferred from one bacterium to
another, is mediated by type IV secretion systems (T4SSs). T4SSs are versatile systems that …

Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA
processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer …

TrwB is the conjugative coupling protein of plasmid R388. TrwBΔN70 contains the soluble
domain of TrwB. It was constructed by deletion of trwB sequences containing TrwB N …

Type IV secretion systems (T4SSs) are bacterial multiprotein organelles specialised in the
transfer of (nucleo) protein complexes across cell membranes. They are essential for …

Bacterial conjugation is a mechanism for horizontal DNA transfer with potential for universal
DNA delivery. The conjugal machinery can be separated into three functional modules: the …

Bacterial conjugation is one of the fundamental processes for gene dissemination or
horizontal gene transfer in nature and involves the transfer of DNA between bacteria in close …

ABSTRACT DNA sequence analysis of five IncW plasmids (R388, pSa, R7K, pIE321, and
pIE522) demonstrated that they share a considerable portion of their genomes and allowed …

Bacterial conjugation is an example of macromolecular trafficking between cells and
responsible for the spreading of antibiotic resistance among bacteria. It involves …

TrwB is an integral membrane protein linking the relaxosome to the DNA transport
apparatus in plasmid R388 conjugation. Native TrwB has been purified in monomeric and …