Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Abstract Marine Rhodobacteraceae (Alphaproteobacteria) are key players of
biogeochemical cycling, comprise up to 30% of bacterial communities in pelagic …

Highlights•13 C-fluxomics provides quantification of metabolic activity in microorganisms.•
Increasing flux analysis capacity has the potential to accelerate strain design.•HT 13 C …

The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …

The genus Pseudomonas comprises approximately 200 species with numerous isolates that
are common inhabitants of soil, water, and vegetation and has been of particular interest for …

Abstract Background The RCA (Roseobacter clade affiliated) cluster belongs to the family
Roseobacteracea and represents a major Roseobacter lineage in temperate to polar …

Polysaccharides are abundant components in Pu-erh tea, yet the utilization of insoluble
polysaccharides under the actions of microbiota has rarely been studied. The aim of this …

Background Glycolysis breakdowns glucose into essential building blocks and ATP/NAD (P)
H for the cell, occupying a central role in its growth and bio-production. Among glycolytic …

Vibrio natriegens is a fast-growing, non-pathogenic bacterium that is being considered as
the next-generation workhorse for the biotechnology industry. However, little is known about …

The core metabolism for glucose assimilation of the soil bacterium and platform strain
Pseudomonas putida KT2440 has been reshaped from the native, cyclically-operating …