Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

Agnostic sequencing for detection of viral pathogens

NPG Gauthier, SD Chorlton, M Krajden… - Clinical microbiology …, 2023 - Am Soc Microbiol
The advent of next-generation sequencing (NGS) technologies has expanded our ability to
detect and analyze microbial genomes and has yielded novel molecular approaches for …

PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens

Z Zhu, R Li, H Zhang, J Wang, Y Lu, D Zhang… - Biosensors and …, 2022 - Elsevier
Pathogenic disease is an important factor affecting rice growth, yield and quality, and the
development and application of rapid diagnostic methods will contribute to the prevention …

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157: H7

SY Lee, SW Oh - Talanta, 2022 - Elsevier
Escherichia coli O157: H7 is a major cause of fresh vegetable-associated infections that can
threaten human health. A method for rapidly detecting food-borne pathogens should be …

Advances in RT-LAMP for COVID-19 testing and diagnosis

G Choi, TJ Moehling, RJ Meagher - Expert Review of Molecular …, 2023 - Taylor & Francis
ABSTRACT Introduction The SARS-CoV-2 pandemic, and the subsequent limitations on
standard diagnostics, has vastly expanded the user base of Reverse Transcription Loop …

Universally stable and precise CRISPR-LAMP detection platform for precise multiple respiratory tract virus diagnosis including mutant SARS-CoV-2 spike N501Y

T Zhang, W Zhao, W Zhao, Y Si, N Chen… - Analytical …, 2021 - ACS Publications
Nowadays, rapid and accurate diagnosis of respiratory tract viruses is an urgent need to
prevent another epidemic outbreak. To overcome this problem, we have developed a …

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

M Xie, T Chen, Z Cai, B Lei, C Dong - Lab on a Chip, 2023 - pubs.rsc.org
Traditional diagnosis of infectious diseases relies on advanced analyzers in central
hospitals, which is not sufficient for rapid control of epidemics, especially in resource-limited …

[HTML][HTML] Loop-mediated isothermal amplification detection of SARS-CoV-2 and myriad other applications

KJM Moore, J Cahill, G Aidelberg… - Journal of …, 2021 - ncbi.nlm.nih.gov
As the second year of the COVID-19 pandemic begins, it remains clear that a massive
increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to …

Toward integrated molecular lateral flow diagnostic tests using advanced micro-and nanotechnology

A Rubio-Monterde, D Quesada-González… - Analytical …, 2022 - ACS Publications
Since the COVID-19 pandemic, some of the routine methods for clinical diagnosis of
infectious diseases have become closer to the general population and terms like …

Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes

EA Phillips, AD Silverman, A Joneja, M Liu… - Nature Biomedical …, 2023 - nature.com
Nucleic acid assays are not typically deployable in point-of-care settings because they
require costly and sophisticated equipment for the control of the reaction temperature and for …