The design of artificial catalysts able to compete with the catalytic proficiency of enzymes is
an intense subject of research. Non-covalent interactions are thought to be involved in …

Microbial enzymes are of great importance in the development of industrial bioprocesses.
Current applications are focused on many different markets including pulp and paper …

The amino-acid sequence of a protein encodes information on its three-dimensional
structure and specific functionality. De novo design has emerged as a method to manipulate …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

In the field of asymmetric synthesis, nonracemic sulfoxides are recognized as valuable and
efficient reagents. Their extensive use as chiral auxiliaries and intermediates is …

Numerous studies have noted that the evolution of new enzymatic specificities is
accompanied by loss of the protein's thermodynamic stability (ΔΔ G), thus suggesting a …

Many research groups successfully rely on whole-gene random mutagenesis and
recombination approaches for the directed evolution of enzymes. Recent advances in …

The use of enzymes and whole bacterial cells has allowed the production of a plethora of
compounds that have been used for centuries in foods and beverages. However, only …

Enzyme catalysis is an inherently dynamic process. Binding and release of ligands is often
accompanied by conformational changes, both subtle and dramatic (reviewed more …

There has been much debate about the extent to which mutational epistasis, that is, the
dependence of the outcome of a mutation on the genetic background, constrains …