Microbes including bacteria are required to respond to their often continuously changing
ecological niches in order to survive. While many signaling molecules are produced as …

Persistent urinary tract infections (UTIs) in hospitalized patients constitute an important
medical problem. It is estimated that 75% of nosocomial UTIs are associated with urinary …

Bacterial biofilms are structured communities of microorganisms that play a critical role in
various industries and healthcare settings, contributing to chronic infections and biofouling …

Nucleotide second messengers are highly versatile signaling molecules that regulate a
variety of key biological processes in bacteria. The best‐studied examples are cyclic AMP …

Monitoring changes of signaling molecules and metabolites with high temporal resolution is
key to understanding dynamic biological systems. Here, we use directed evolution to …

Pseudomonas aeruginosa is a cause of chronic respiratory tract infections in people with
cystic fibrosis (CF), non-CF bronchiectasis, and chronic obstructive pulmonary disease …

The ubiquitous bacterial second messenger cyclic diguanylate (c-di-GMP) coordinates
diverse cellular processes through its downstream receptors. However, whether c-di-GMP …

The Pel exopolysaccharide is one of the most mechanistically conserved and
phylogenetically diverse bacterial biofilm matrix determinants. Pel is a major contributor to …

Many bacteria use the second messenger c-di-GMP to regulate exopolysaccharide
production, biofilm formation, motility, virulence, and other phenotypes. The c-di-GMP level …

Confronting the challenge of biofilm resistance and widespread antimicrobial resistance
(AMR), this study emphasizes the need for innovative monitoring methods and explores the …