Large-scale computational molecular models provide scientists a means to investigate the
effect of microscopic details on emergent mesoscopic behavior. Elucidating the relationship …

Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

Label-free characterization of single biomolecules aims to complement fluorescence
microscopy in situations where labeling compromises data interpretation, is technically …

The use of imaging technologies has been critical in deciphering biological phenomena,
structures, and mechanisms across a wide range of spatial scales. The spatial resolution of …

Localization of single fluorescent emitters is key for physicochemical and biophysical
measurements at the nanoscale and beyond ensemble averaging. Examples include single …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …

Biological systems are dynamical, constantly exchanging energy and matter with the
environment in order to maintain the non-equilibrium state synonymous with living …

Visualizing and manipulating the behavior of proteins is crucial to understanding the
physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain …

Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Fluorescence microscopy has become a critical tool for researchers to understand biological
processes at the cellular level. Micrographs from fixed and live-cell imaging procedures …